Clinical application of polymerase chain reaction amplification to diagnosis of herpes virus infection.
Clinical application of polymerase chain reaction amplification to diagnosis of herpes virus infection.
复制标题
聚合酶链反应扩增技术在疱疹病毒感染诊断中的临床应用
DOI：
10.1097/00000372-199406000-00007
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Feldman,SR
中科院分区：
文献类型：
--
作者：
Thomas,CA;Smith,SE;Morgan,TM;White,WL;Feldman,SR
Amplification of viral DNA offers a potentially sensitive and specific method for identifying herpes viruses in pathologic specimens. The purpose of this study is to assess the value of polymerase chain reaction amplification of DNA as a diagnostic test for herpes virus in pathologic specimens. The purpose of this study is to assess for herpes virus infections. We examined 79 paraffinembedded tissue samples from 43 patients and 55 viral culture samples from 45 patients. Herpes virus DNA in the specimens was amplified by polymerase chain reaction. Using paraffin-embedded tissue on which a diagnosis of herpes virus was made by morphologic criteria, 11 of 19 patients had herpes virus DNA identified by PCR; herpes virus DNA was not identified in any of 35 negative control specimens. Herpes virus DNA was also identified by polymerase chain reaction in all of the positive herpes virus culture specimens. Of 29 culture negative specimens, herpes virus DNA was identified in six. We conclude that polymerase chain reaction is useful to establish or confirm the presence of a herpes virus infection in paraffin-embedded tissue samples and that it is more sensitive than viral culture.
