The role of AV2 (''precoat'') and coat protein in viral replication and movement in tomato leaf curl geminivirus
The role of AV2 (''precoat'') and coat protein in viral replication and movement in tomato leaf curl geminivirus
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DOI：
10.1006/viro.1996.0546
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发表时间：
1996-10-15
期刊：
影响因子：
3.7
通讯作者：
Fauquet, CM
中科院分区：
文献类型：
--
作者：
Padidam, M;Beachy, RN;Fauquet, CM
We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deletions in AV2 developed very mild symptoms and accumulated only low levels oi both single-stranded (ss) and double-stranded (ds) viral DNA, whereas inoculated protoplasts accumulated both ss and dsDNA to wild-type levels, showing that AV2 is required for efficient viral movement. However, both plants and protoplasts inoculated with substitution, frameshift, and other similar mutations in AV2 accumulated low levels of viral DNA. The low levels oi accumulation of DNA of these mutants were apparently not due to a defect in AV2 synthesis. Mutations in the CP caused a marked decrease in ssDNA accumulation in plants and protoplasts while increasing dsDNA accumulation in protoplasts, Mutations in both AV2 and CP behaved like AV2 mutants in plants and like CP mutants in protoplasts. The results demonstrated that multiple functions provided by AV2, BV1, and BC1 are essential for viral movement, and that changes in A-component virion-sense mRNA structure or translation affect viral replication. (C) 1996 Academic Press, Inc.
