Attraction of Brachyspira pilosicoli to mucin
Attraction of Brachyspira pilosicoli to mucin
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DOI：
10.1099/mic.0.030262-0
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发表时间：
2010-01-01
期刊：
影响因子：
2.8
通讯作者：
Hampson, David J.
中科院分区：
文献类型：
--
作者：
Naresh, Ram;Hampson, David J.
厌氧肠道螺旋体Brachyspira pilosicoli定植于包括人类在内的各种物种的大肠。在结肠中，这种螺旋体可以穿透上面的粘液层，通过一个细胞末端附着在下面的肠上皮细胞上，并引发局部结肠炎和腹泻。本研究的目的是调查是否，作为殖民过程的一部分，B。纤毛被粘蛋白吸引。B B 15在体外毛细管测定中，分析了从人、猪、鸡和狗分离的pilosicoli菌株和猪短螺旋体的对照菌株进入猪胃粘蛋白溶液的能力。用1%粘蛋白没有检测到显著的吸引力，但是一些菌株开始进入2%溶液，然后吸引力随着浓度的增加而增加，在约6- 8%粘蛋白处达到峰值。在B中观察到类似的增加。hyoacetteriae，尽管这种活性在6%粘蛋白时达到峰值，然后下降，表明这两个物种对粘蛋白具有不同的亲和力。这些粘蛋白浓度远高于先前用短螺旋体属物种进行的实验研究中使用的浓度。6- 8%粘蛋白溶液的粘度为约7-12 mPa·s，这与实验猪的盲肠和结肠中覆盖上皮的粘液层的测量粘度相似。菌株的运动性不同，评估其进入含有趋化性缓冲液的管的能力，但这种运动性和它们进入粘蛋白溶液的能力的程度之间没有显着的关系。不同的菌株也有不同的倾向，进入粘蛋白的解决方案，但没有一致的差异，根据宿主物种的起源。B。pilosicoli菌株95/1000被吸引向D-丝氨酸的溶液，表明趋化性参与对粘蛋白的吸引;然而，95/1000也被吸引到聚乙烯吡咯烷酮（PVP）的粘性溶液，以反映对粘蛋白的响应的方式，因此表明趋粘性参与对粘蛋白的吸引。B。hyoacetteriae B204的趋粘性与PVP相似。需要进一步的研究来确定给定菌株与粘蛋白的体外相互作用是否是其体内定殖能力的有用指标，因此可以用作毒力的潜在标记。
The anaerobic intestinal spirochaete Brachyspira pilosicoli colonizes the large intestine of various species, including humans. In the colon this spirochaete can penetrate the overlying mucus layer, attach by one cell end to the underlying enterocytes, and initiate localized colitis and diarrhoea. The aim of this study was to investigate whether, as part of the colonization process, B. pilosicoli is attracted to mucin. Fifteen B. pilosicoli strains isolated from humans, pigs, chickens and dogs, and a control strain of Brachyspira hyodysenteriae, were analysed for their ability to enter solutions of hog gastric mucin in an in vitro capillary tube assay. No significant attraction was detected with 1 % mucin, but some strains started to enter a 2 % solution, and attraction then increased with increasing concentrations to peak at around 6-8 % mucin. A similar increase was seen with B. hyodysenteriae, although this activity peaked at 6% mucin and then declined, suggesting that the two species have different affinities for mucin. These mucin concentrations were much higher than those used in previous experimental studies with Brachyspira species. The viscosities of the 6-8 % mucin solutions were around 7-12 mPa s, which were similar to the measured viscosities of the mucus layer overlying the epithelium in the caecum and colon of experimental pigs. The strains varied in their motility, as assessed by their ability to enter tubes containing chemotaxis buffer, but there was no significant relationship between this motility and the extent of their ability to enter the mucin solutions. Different strains also had different propensities to enter the mucin solutions, but there were no consistent differences according to the host species of origin. B. pilosicoli strain 95/1000 was attracted towards a solution of D-serine, suggesting that chemotaxis was involved in the attraction to mucin; however, 95/1000 was also attracted to viscous solutions of polyvinylpyrrolidone (PVP), in a manner mirroring the response to mucin, and hence suggesting the involvement of viscotaxis in the attraction to mucin. B. hyodysenteriae B204 showed a similar viscotaxis to PVP. Further studies are required to determine whether the in vitro interaction of a given strain with mucin is a useful indicator of its in vivo colonization ability, and hence could be used as a potential marker for virulence.