Hebbian Plasticity Guides Maturation of Glutamate Receptor Fields In Vivo
Hebbian Plasticity Guides Maturation of Glutamate Receptor Fields In Vivo
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DOI：
10.1016/j.celrep.2013.04.003
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发表时间：
2013-05-01
期刊：
影响因子：
8.8
通讯作者：
Kittel, Robert J.
中科院分区：
文献类型：
--
作者：
Ljaschenko, Dmitrij;Ehmann, Nadine;Kittel, Robert J.
突触可塑性塑造了功能性神经回路的发展，并为学习和记忆的细胞模型提供了基础。赫布可塑性描述了突触强度的活动依赖性变化，这种变化是输入特异性的，并取决于相关的突触前和突触后活动。虽然人们认识到突触活动和突触发育是密切相关的，但我们对这种耦合的机制的理解还远远没有完成。利用视紫红质-2在体内诱发活动，我们研究了果蝇神经肌肉接头的突触可塑性。值得注意的是，相关的突触前和突触后刺激通过促进突触特异性招募GluR-IIA型谷氨酸受体亚基进入突触后受体领域来增加突触后敏感性。相反，GluR-IIA被迅速从活动未能引起大量突触后去极化的突触中去除。将这些结果与发育GluR-IIA动力学相结合，提供了一个全面的生理概念，即赫布可塑性如何指导突触成熟和稀疏递质释放控制单个突触分子组成的稳定。
Synaptic plasticity shapes the development of functional neural circuits and provides a basis for cellular models of learning and memory. Hebbian plasticity describes an activity-dependent change in synaptic strength that is input-specific and depends on correlated pre- and postsynaptic activity. Although it is recognized that synaptic activity and synapse development are intimately linked, our mechanistic understanding of the coupling is far from complete. Using Channelrhodopsin-2 to evoke activity in vivo, we investigated synaptic plasticity at the glutamatergic Drosophila neuromuscular junction. Remarkably, correlated pre- and postsynaptic stimulation increased postsynaptic sensitivity by promoting synapse-specific recruitment of GluR-IIA-type glutamate receptor subunits into postsynaptic receptor fields. Conversely, GluR-IIA was rapidly removed from synapses whose activity failed to evoke substantial postsynaptic depolarization. Uniting these results with developmental GluR-IIA dynamics provides a comprehensive physiological concept of how Hebbian plasticity guides synaptic maturation and sparse transmitter release controls the stabilization of the molecular composition of individual synapses.