Isolation and characterization of native activin B.
Isolation and characterization of native activin B.
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天然激活素 B 的分离和表征。
DOI：
10.1016/s0021-9258(18)42014-5
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hiromu Sugino
中科院分区：
文献类型：
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作者：
Takanori NakamuraS;Makoto Asashimas;Yuzuru EtolI;Koji Takios;Hideho UchiyamaQ;Naomi MoriyaQ;Takashi AriizumiQ;Takayuki YashiroQ;Kishiko SuginoS;Koiti TitaniSII;Hiromu Sugino
为了检查激活素是否与卵泡抑素（一种激活素结合蛋白）结合以在体内形成复合物，我们尝试从猪卵泡液中纯化激活素-卵泡抑素复合物。由此获得的结果表明，几乎等摩尔量的激活素A、AB和B以与卵泡抑素的复合物形式存在于卵泡液中。纯化复合物的反相高效液相色谱产生卵泡抑素和激活素 A、AB 和 B。在各种测定系统中，例如刺激卵泡刺激素分泌、诱导红细胞分化和增强卵巢细胞上促性腺激素受体的表达，发现纯化的激活素 B 的活性显着低于其他激活素的活性。此外，125I-激活素A与激活素响应的红白血病细胞的结合被激活素B竞争，其效力比其他激活素低约10倍。与这些结果相反，激活素 B 被证明具有与其他激活素相当的有效非洲爪蟾中胚层诱导活性。这表明，与激活素A和AB不同，激活素B只能引发中胚层诱导活性，而不能在其他生物系统中发挥作用，这表明激活素B在早期发育和未知的生物学功能中具有特定作用。
To examine whether activin binds to follistatin, an activin-binding protein, to form a complex in vivo, we attempted to purify activin-follistatin complex from porcine follicular fluid. Our results thus obtained indicated that almost equimolar amounts of activins A, AB, and B are present as a complex with follistatin in the follicular fluid. Reverse-phase high performance liquid chromatography of the purified complex yielded follistatin and activins A, AB, and B. The activity of the purified activin B was found to be significantly lower than those of other activins in various assay systems such as stimulation of follicle-stimulating hormone secretion, induction of erythrodifferentiation, and potentiation of expression of gonadotropin receptors on ovarian cells. Moreover, binding of 125I-activin A to erythroleukemic cells which are activin-responsive was competed by activin B with approximately 10-fold lower potency compared with other activins. In contrast to these results, activin B was proved to have a potent Xenopus mesoderm-inducing activity, comparable with that of other activins. This indicates that, unlike activins A and AB, activin B can only elicit mesoderm-inducing activity and cannot function in other biological systems, suggesting a specific role of activin B in early development and unknown biological functions.