A Rat Tibial Growth Plate Injury Model to Characterize Repair Mechanisms and Evaluate Growth Plate Regeneration Strategies
A Rat Tibial Growth Plate Injury Model to Characterize Repair Mechanisms and Evaluate Growth Plate Regeneration Strategies
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DOI：
10.3791/55571
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发表时间：
2017-07-01
影响因子：
1.2
通讯作者：
Payne, Karin A.
中科院分区：
文献类型：
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作者：
Erickson, Christopher B.;Shaw, Nichole;Payne, Karin A.
三分之一的儿童骨折涉及生长板，并可能导致骨骼生长受损。生长板(或生长板)是一种软骨组织，位于儿童所有长骨的末端，负责纵骨生长。一旦受损，生长板内的软骨组织可能会过早骨化，导致不需要的骨修复组织，形成一根“骨条”。在某些情况下，这种骨条可能会导致骨生长畸形，如角畸形，或者它可以完全阻止纵向骨生长。目前还没有临床治疗方法可以完全修复受损的生长板。使用生长板损伤的动物模型来更好地了解骨条形成的机制并确定抑制它的方法是开发更好的生长板损伤治疗方法的一个很好的机会。该方案描述了如何使用钻孔缺陷破坏大鼠胫骨近端生长板。这种小动物模型可以可靠地产生骨条，并可能导致类似于儿童的生长畸形。该模型允许研究骨条形成的分子机制，并作为测试生长板损伤潜在治疗方案的一种手段。
A third of all pediatric fractures involve the growth plate and can result in impaired bone growth. The growth plate (or physis) is cartilage tissue found at the end of all long bones in children that is responsible for longitudinal bone growth. Once damaged, cartilage tissue within the growth plate can undergo premature ossification and lead to unwanted bony repair tissue, which forms a "bony bar." In some cases, this bony bar can result in bone growth deformities, such as angular deformities, or it can completely halt longitudinal bone growth. There is currently no clinical treatment that can fully repair an injured growth plate. Using an animal model of growth plate injury to better understand the mechanisms underlying bony bar formation and to identify ways to inhibit it is a great opportunity to develop better treatments for growth plate injuries. This protocol describes how to disrupt the rat proximal tibial growth plate using a drill-hole defect. This small animal model reliably produces a bony bar and can result in growth deformities similar to those seen in children. This model allows for investigation into the molecular mechanisms of bony bar formation and serves as a means to test potential treatment options for growth plate injuries.