Existence of high abundance antiproliferative mRNA's in senescent human diploid fibroblasts.
Existence of high abundance antiproliferative mRNA's in senescent human diploid fibroblasts.
复制标题
衰老人二倍体成纤维细胞中存在高丰度抗增殖 mRNA。
DOI：
10.1126/science.2421407
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Smith,JR
中科院分区：
文献类型：
--
作者：
LumpkinJr,CK;McClung,JK;Pereira-Smith,OM;Smith,JR
从衰老的人二倍体成纤维细胞（HDF）中分离的聚腺苷化RNA在微注射后抑制了增殖能力细胞的DNA合成，而从年轻的HDF中分离的聚腺苷化RNA则没有抑制作用。通过去除血清生长因子使年轻细胞处于静止状态的聚腺苷化RNA对DNA合成有轻微的抑制作用。来自衰老细胞的抑制剂信使RNA （mRNA）丰度估计为0.8，而静止细胞的丰度估计为0.005%。这些结果表明，在非增殖的正常人类细胞中存在一种或多种抗增殖mRNA；这些RNA编码的因子要么对DNA合成的启动子起拮抗作用，要么以某种方式调节启动子的表达。衰老细胞中抑制mRNA的丰度水平表明开发互补DNA探针的可行性，这将有助于研究细胞周期控制机制。
Polyadenylated RNA isolated from senescent human diploid fibroblasts (HDF) inhibited DNA synthesis in proliferation-competent cells after microinjection, whereas polyadenylated RNA from young HDF had no inhibitory effect. Polyadenylated RNA from young cells made quiescent by removal of serum growth factors had a slight inhibitory effect on DNA synthesis. The abundance level of inhibitor messenger RNA (mRNA) from senescent cells was estimated at 0.8 and that of quiescent cells at 0.005 percent. These results demonstrate the existence of one or more antiproliferative mRNA's in nonproliferating normal human cells; these RNA's code for factors that either work antagonistically to initiators of DNA synthesis or regulate the expression of the initiators in some way. The abundance level of the inhibitory mRNA in senescent cells indicates the feasibility of developing a complementary DNA probe that will be useful in studying cell cycle control mechanisms.