Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA.
Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA.
复制标题
人甲状腺过氧化物酶微粒体抗原 cDNA 的完整核苷酸序列。
DOI：
10.1093/nar/15.16.6735
复制
发表时间：
1987
影响因子：
14.9
通讯作者：
C. Dinsart
中科院分区：
文献类型：
--
作者：
F. Libert;J. Ruel;M. Ludgate;S. Swillens;N. Alexander;G. Vassart;C. Dinsart
使用多克隆抗猪过氧化物酶抗体（由J. Pomeland和J. Kaniewski-Paris赠送）从AgtII甲状腺cDNA文库中分离出2kb的人甲状腺过氧化物酶cDNA克隆。序列分析显示与猪甲状腺过氧化物酶部分序列有68%的同源性（1）。通过杂交重新筛选文库允许分离完整的cDNA，从其推导出以下序列。00 A0 TA 0A 0 AA 000 000 CT * O 0AGA 0 0AAAAA AOCAOW 0 000000000 00 AT 0 CTCy0_A 0 C 0100 OCC？TT0OCA0ICTS* 00GM A0*£ CT0CTT 20000 000* 091 00000000 A0000CT0 A00AAAA OCOICTOOGM GACACOOCCA 0200 00TAC 0OC GAT 20A £ 090 A90010* 90 AAA0A？T 00I0IC000 00c00C IGCTTrrmC cAAAcTTCCT aOAA0cAA0cMamT 0300 T0COA OCA900AT£ T？00£ 9A£ C* TACAliO ATGAAA*£* 91* 0CCT Q* UATAA C£ TCACAaC AT£ AAC* A tOCTTTATCA 0400 OAA0ATCTOC T0AWCAT TOCAAA0 TC00T0 0000A* ATw0000 AAToOC ATAc0000 000AATACkAOOCA 0600* CAOOA0C TOCAACAAC 900000CC CCA0* O00 00CkCCAAC£ 00C2TM0 CAC00T* T00 0 0 AM TAT 00900 £ 0 OCT* 0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000 OOCATACA£ C 000000_00 000000000 0900 00CCT0CTAC COOOC 00000200l00 C0* 00000* CAAlOCOOO ICTT 000 * O000000 OCCAAO 7cwOC0CAGT 0* AA000 1000 ACCT00000OA 000 U0C C0* 00AT 00CA0C00 090000£ 0A£ MA0£ 0 0C00CT0U 0CAO0M A000C2 00 1100 000010 00£ COOCCTC000AT0 00090ACC 00C00 OWCA0W0 0C00002000 00000 £ DC 00 000 00990 ATCOO9OO 0 1200 000C021 CT20000G AC000C00 CA* 0k900 00000O0 00W01O2A C0C9002 00CT00090 AC0A 0000 1300 CTC£ 0 TC12M00AC£ 0000 0900W002 ACC£ 0900 00£ AA£ OTC 020C 2ACAT CATCAk 02 * A0-TTACA 1400 T1COCA90AT C002000900 090000000 A9CA000tOT 0000CC0TAT 0A900CTATO AC10CC00 CA0C900ACT 020CA 200 1500 A900000T0C A200CACT 0900CCk0 020900900 T200C9009 00900 c09000090 T2CCk000 0200C020C 1600C0900010T TC1I100dhT2 0£ CATTA 1 00T0902 000000 ACT 9 £ t£ 90 0000T1C000 CAA* AC0A0 CAAAC 0CA 9 00A0tC 1700 A0CT0A2£ AA 0G090900T0 ACC£ AAA0 10TT0002C0 010CAAT1OC90CA 9T02A ££ A102000X C00CA* 9002 0000090 1800 ZI002090T TACA* T2A0T 00A9000901 000200000 909CC0C 2090000900 AC90tC0 CC00A90909 00200009 1900* tOC1O ACT0TA0CAA0CATCC£ 0£ CA£ CAT90 ATO T1000 A900190 T AAAACT0C00 1090 10900 0202 2000 0020010T000* A00A0 amcc9000TT TOo2wI0A002 2T090200002 A 0CACCtCT9CA00AT9C0C0C 020A0T 2100 0A90C900C 2C0000 1CA012 CA* 9C0W9 C0C0CCA002000T * 20 0 00CCAtAT tCOC002A90 CT000A0T91 2200 T2TG0A29CA TC100£ AT 09200T90 00C000AAAC £ Trf1c 10A99009* 90202000 T10900 CAT 0 000* A002M0 2300 20090220* 00901T0 9G0C0020 T2W0ATTC C000CCC00009 OATGA0 TCCA0W* 0 0WA0900001 9C00AC090C9G0 2400* 0ATT0A00000= TCT1 0CAAAATGtC00C0920 0902C0 09C00CT200AC000 1C2 0 CA00 AACAC
A human thyroid peroxidase cDNA clone of 2 kb was isolated from a Agtll thyroid cDNA library using a polyclonal antiporcine peroxidase antibody (gift of J. Pommier and J. Kaniewski-Paris). Sequence analysis showed 68% homology with the partial porcine thyroid peroxidase sequence (1). Re-screening of the library by hybridization allowed isolation of the complete cDNA from which the following sequence was deduced.00 A0TA0A0 AA000 000CT* O0AGA0 0AAAAA AOCAOW0 000000 0000000 00AT0 CTCy0_A 0C 0100 OCC? TT0OCA0ICTS* 00GM A0*£ CT0CTT 20000 000* 091 00000000 A0000CT0 A00AAAA OCOICTOOGM GACACOOCCA 0200 00TAC0OC GAT20A£ 090 A90010* 90 AAA0A? T 00I0IC000 00c00C IGCTTrrmC cAAAcTTCCT aOAA0cAA0cMamT 0300 T0COA OCA900AT£ T? 00£ 9A£ C* TACAliO ATGAAA*£* 91* 0CCT Q* UATAA C£ TCACAaC AT£ AAC* A tOCTTTATCA 0400 OAA0ATCTOC T0AWCAT TOCAAA0 TC00T0 0000A* ATw0000 AAToOC ATAc0000 000AATACkAOOCA 0600* CAOOA0C TOCAACAAC 900000CC CCA0* O00 00CkCCAAC£ 00C2TM0 CAC00T* T00 0 AM TAT00900£ 0 OCT* CAA 06B00 00000* 000C 000AACC* 00 0WT00T0A CAAOOOOOCACOGOOC C0000000000009ACA0AA CAT0COATTC AkOTTCAAA l 0700 ACAUT0T0 AkOOCTATTC T090C000 AT* 0T0 0 GCTACAT 000CCA09£ 100MAC£ CkCCA9CA0M CA0AOCAOA 06100 _oooAOOo 0AT£ C0CA00 AT0ACT00 A9ACCAA CO0A? OOCATACA£ C 000000_00 00000000 00000000 0900 00CCT0CTAC COOOC00000200l00 C0* 00000* CAAlOCOOO ICTT000* O0000000 OCCAAO 7cwOC0CAGT 0* AA000 1000 ACCT00000OA000 U0C C0* 00AT 00CA0C00 090000£ 0A£ MA0£ 0 0C00CT0U 0CAO0M A000C2 00 1100 000010 00£ COOOCCTC000AT0 00090ACC 000C00 OWCA0W0 0C00002000 000000£ DC 00 000 00990 ATCOO9OO 0 1200 000C021 CT20000G AC000C00 CA* 0k900 00000O0 00W0lO2A C0C9002 00CT000090 AC0A 0000 1300 CTC£ 0 TC12M00AC£ 0000 0900W002 ACC£ 0900 00£ AA£ OTC 020C 2ACAT CATCAk02* A0£ TTACA 1400 T1COCA90AT C002000900 0900000000 A9CA000tOT 0000CC0TAT 0A900CTATO AC10CC00 CA0C900ACT 020CA 200 1500 A900000T0C A200CACT 0900CCk0 020900900 T200C9009 00900 c090000090 T2CCk000 0200C020C 1600C0900010T TC1I1OOdhT2 0£ CATTA1 00T0902 0000000 ACT9£ t£ 90 0000T1C000 CAA* AC0A0 CAAAC0CA9 00A0tC 1700 A0CT0A2£ AA 0G090900T0 ACC£ AAA0 10TT0002C0 010CAAT1OC90CA9T02A££ A102000X C00CA* 9002 0000090 1800 ZI002090T TACA* T2A0T 00A9000901 000200000 909CC0C 2090000900 AC90tC0 CC00A90909 00200009 1900* tOC1O ACT0TA0CAA0CATCC£ 0£ CA£ CAT90ATO T1000 A900190T AAAACT0C00 1090 10900 0202 2000 0020010T000* A00A0 amcc9000TT TOo2wI0A002 2T090200002 A 0CACCtCT9CA00AT9C0C0C 020A0T 2100 0A90C900C 2C0000 1CA012 CA* 9C0W9 C0C0CCA0020000T* 20 0 00CCAtAT tCOC002A90 CT000A0T91 2200 T2TG0A29CA TC100£ AT 09200T90 00C0000 AAAC£ Trf1c 10A99009* 90202000 T10900 CAT 0 000* A002M0 2300 20090220* 00901T0 9G0C0020 T2W0ATTC C000CCC00009 OATGA0 TCCA0W* 0 0WA0900001 9C00AC090C9G0 2400* 0ATT0A00000= TCT1 0CAAAATGtC00C0920 0902C0 09C00CT200AC000 1C2 0 CA00 AACAC