Serum amyloid A transcription in Atlantic salmon (Salmo salar L.) hepatocytes is enhanced by stimulation with macrophage factors, recombinant human IL-1β, IL-6 and TNFα or bacterial lipopolysaccharide
Serum amyloid A transcription in Atlantic salmon (Salmo salar L.) hepatocytes is enhanced by stimulation with macrophage factors, recombinant human IL-1β, IL-6 and TNFα or bacterial lipopolysaccharide
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DOI：
10.1016/s0145-305x(00)00022-7
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Robertsen, B
中科院分区：
文献类型：
--
作者：
Jorgensen, JB;Lunde, H;Robertsen, B
血清淀粉样蛋白A （B-SAA）曾被报道为鲑科鱼类的急性期蛋白。肝细胞是鲑科鱼类中a -SAA的主要来源，但对鱼类中肝细胞SAA的合成尚不清楚。本研究采用Northern blot方法研究了A-SAA转录本在大西洋鲑鱼肝细胞原代培养物中对巨噬细胞源性细胞因子、人重组细胞因子和细菌脂多糖（LPS）的表达。用LPS、酵母葡聚糖或白细胞源性巨噬细胞活化因子（MAF）刺激大西洋鲑鱼头肾巨噬细胞制备巨噬细胞上清液。葡聚糖和maf刺激的巨噬细胞上清液对肝细胞a - saa的表达没有影响，而lps刺激的巨噬细胞上清液的表达增加了约2倍。葡聚糖与MAF或LPS与MAF联合使用效果更好，这些上清液分别使a - saa表达增加3.4倍和5.2倍。肝细胞也用人重组细胞因子TNF α、IL-1 β和IL-6单独或联合处理。a - saa单独对它们的反应一般，但TNF - α和IL-6或IL-1 β和IL-6联合使用比单独使用每种细胞因子的反应更高。这些数据表明大西洋鲑鱼肝细胞A-SAA的表达是由细胞因子样分子诱导的。有趣的是，直接用LPS处理的肝细胞使SAA mRNA表达增加了10倍以上，但尚不清楚这是LPS对肝细胞的直接影响还是由其他污染细胞类型介导的。(C) 2000 Elsevier Science Ltd.版权所有。
Serum amyloid A (B-SAA) has previously been reported to be an acute-phase protein in salmonids. Hepatocytes represent a major source of A-SAA in salmonids, but nothing is known about hepatocyte SAA synthesis in fish. In the present work, the expression of A-SAA transcripts in primary cultures of Atlantic salmon hepatocytes in response to macrophage derived cytokines, human recombinant cytokines and bacterial lipopolysaccharide (LPS) was studied by Northern blot analysis. The macrophage supernatants were prepared by stimulating Atlantic salmon head kidney macrophages with LPS, yeast glucan or a leukocyte derived macrophage activating factor (MAF). The supernatants from glucan- or MAF-stimulated macrophages had no effect on A-SAA expression of the hepatocytes while supernatants from LPS-stimulated macrophages gave about a 2-fold increase in expression. The combination of either glucan and MAF, or LPS and MAF were more effective and these supernatants gave a 3.4- and 5.2-fold increase in A-SAA expression, respectively. The hepatocytes were also treated with the human recombinant cytokines TNF alpha, IL-1 beta and IL-6, alone or in combination. The A-SAA response to each of them alone was modest, but TNF alpha and IL-6 or IL-1 beta and IL-6 in combination gave a higher response than each cytokine alone. These data suggest that the expression of A-SAA by hepatocytes from Atlantic salmon is induced by cytokine-like molecules. Interestingly, hepatocytes treated directly with LPS gave a more than 10-fold increase in SAA mRNA expression, but it is not known if this is a direct effect of LPS on the hepatocytes or if it is mediated by other contaminating cell types. (C) 2000 Elsevier Science Ltd. All rights reserved.