The cumulative activity of benzo(a)pyrene on systemic immune responses with mite allergen extract after intranasal instillation and ex vivo response to ovalbumin in mice
The cumulative activity of benzo(a)pyrene on systemic immune responses with mite allergen extract after intranasal instillation and ex vivo response to ovalbumin in mice
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DOI：
10.1016/j.toxlet.2005.01.002
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发表时间：
2005-05-16
影响因子：
3.5
通讯作者：
Kazemnejad, A
中科院分区：
文献类型：
--
作者：
Kadkhoda, K;Pourfathollah, AA;Kazemnejad, A
本文研究了柴油机尾气颗粒中存在的多环芳烃苯并(a)芘（BP）经鼻内滴入和体外暴露于过敏原后对小鼠全身免疫反应的累积活性。主要目的是阐明BP在这种反应中是否有辅助作用。用螨过敏原提取物（MAE）单独或联合BP免疫Balb/cA雄性小鼠。对照组仅给予BP或缓冲液。这些动物接种了四次免疫。末次免疫1周后，采用ELISA法检测血清中抗mae IgE抗体及同一过敏原再攻后脾细胞分泌的IL-4、IL- 10、IL-12p70和ifn - γ。与单独接受MAE的动物相比，接受MAE和BP的动物对抗原的反应增加。在另一项尝试中，从幼年雄性Balb/c小鼠中获得骨髓来源的树突状细胞（DCs），并暴露于不同剂量的卵清蛋白（OA）和BR。研究分为四组：对照组、BP组、OA组和OA + BP组。与单独接受OA的动物相比，接受OA和BP的动物的反应升高。综上所述，本研究表明，BP在鼻内滴入后对特异性IgE和Th1/Th2细胞因子的产生以及体外暴露后dc分泌细胞因子具有累积活性，且这种现象具有剂量依赖性。因此，这可能是树突状细胞免疫调节作用的又一证据。2005爱思唯尔爱尔兰有限公司版权所有。
The cumulative activity of benzo(a)pyrene (BP) as one of the polycyclic aromatic hydrocarbons which is present in diesel exhaust particles on systemic immune responses was studied in mice after intranasal instillation and in vitro exposure to allergens. The main purpose was to elucidate whether BP has an adjuvant effect in this responses or not. Male Balb/cA mice were immunized with mite allergen extract (MAE) either alone or in combination with BP. Controls were given BP, or buffer alone. The animals were immunized four times. One week after the last immunization, anti-MAE IgE antibody in serum and IL-4, IL- 10, IL-12p70 and IFN-gamma secreted by spleen cells upon rechallenge with the same allergen, was measured by ELISA. An increased response to the antigen was observed in animals receiving MAE together with BP, compared with animals receiving MAE alone. In another attempt, bone marrow-derived dendritic cells (DCs) are obtained from naive male Balb/c mice and were exposed to the varying doses of ovalbumin (OA) and BR There were four groups of study: control, BP, OA and OA plus BP. An elevated response was observed in animals receiving OA together with BP, compared with animals receiving OA alone.In conclusion, this work shows that BP has an cumulative activity for specific IgE and Th1/Th2 cytokines production after intranasal instillation and cytokine secretion by DCs after in vitro exposure, and this phenomenon is dose-dependent. Thus, it may be another evidence on immunomodulatory effect of dendritic cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved.