Structural analysis of xyloglucans in the primary cell walls of plants in the subclass Asteridae
Structural analysis of xyloglucans in the primary cell walls of plants in the subclass Asteridae
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DOI：
10.1016/j.carres.2005.04.016
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发表时间：
2005-08-15
影响因子：
3.1
通讯作者：
York, WS
中科院分区：
文献类型：
--
作者：
Hoffman, M;Jia, ZH;York, WS
对几种菊科植物木葡聚糖的结构进行了研究，以确定其在不同分类目中的结构差异。通过连续的（酶和化学）提取过程从植物细胞壁中分离得到木葡聚糖，并通过核磁共振光谱和质谱分析对其结构进行了表征。所有被检测的树属植物，包括莴苣（莴苣目）、青豆（豆荚目）和胡萝卜（胡萝卜目），都产生典型的木葡聚糖，具有xxx型分支模式，含有α - d - xylp -、β - d - xylp -(1 ->)- α - l - fucp -(1 -> 2)- β - d - galp -(1 -> 2)- α - d - xylp -侧链。然而，层状体产生非典型木葡聚糖。例如，先前的分析表明，辣椒（辣椒）和番茄（番茄）这两个属茄属目的物种，以及橄榄（橄榄，橄榄目）产生的木葡聚糖含有阿拉伯糖基和半乳糖基残基，但缺乏焦酰基残基。茄属植物生产的xxgg型木葡聚糖比油橄榄生产的xxgg型木葡聚糖分支较少。本研究表明，Ipomoea pupurea（牵牛花，茄属）、Ocimum basilicum（罗勒，叶状植物目）和Plantago major（车前草，叶状植物目）都产生缺乏facosyl残基的木葡聚糖，并且具有不寻常的xxggg型分支模式，其中基本重复核心在主干中包含五个葡萄糖亚基。此外，Neruim夹竹桃（龙胆亚目）产生含有阿拉伯糖基、半乳糖基和焦酰基残基的xxx型木葡聚糖。夹竹桃中这种中间木聚糖结构的出现与木聚糖结构的进化发展及其在植物初生细胞壁中的作用有关。(c) 2005 Elsevier Ltd版权所有。
The structures of xyloglucans from several plants in the subclass Asteridae were examined to determine how their structures vary in different taxonomic orders. Xyloglucans, solubilized from plant cell walls by a sequential (enzymatic and chemical) extraction procedure, were isolated, and their structures were characterized by NMR spectroscopy and mass spectrometry. All campanulids examined, including Lactuca sativa (lettuce, order Asterales), Tenacetum ptarmiciflorum (dusty miller, order Asterales), and Daucus carota (carrot, order Apiales), produce typical xyloglucans that have an XXXG-type branching pattern and contain alpha-D-Xylp-, beta-D-Galp-(1 -> 2)-alpha-D-Xylp-, and alpha-L-Fucp-(1 -> 2)-beta-D-Galp-(1 -> 2)-alpha-D-Xylp- side chains. However, the lamiids produce atypical xyloglucans. For example, previous analyses showed that Capsicum annum (pepper) and Lycopersicon esculentum (tomato), two species in the order Solanales, and Olea europaea (olive, order Lamiales) produce xyloglucans that contain arabinosyl and galactosyl residues, but lack fucosyl residues. The XXGG-type xyloglucans produced by Solanaceous species are less branched than the XXXG-type xyloglucan produced by Olea europaea. This study shows that Ipomoea pupurea (morning glory, order Solanales), Ocimum basilicum (basil, order Lamiales), and Plantago major (plantain, order Lamiales) all produce xyloglucans that lack facosyl residues and have an unusual XXGGG-type branching pattern in which the basic repeating core contains five glucose subunits in the backbone. Furthermore, Neruim oleander (order Gentianales) produces an XXXG-type xyloglucan that contains arabinosyl, galactosyl, and fucosyl residues. The appearance of this intermediate xyloglucan structure in oleander has implications regarding the evolutionary development of xyloglucan structure and its role in primary plant cell walls. (c) 2005 Elsevier Ltd. All rights reserved.