Basal Cell-Extracellular Matrix Adhesion Regulates Force Transmission during Tissue Morphogenesis
Basal Cell-Extracellular Matrix Adhesion Regulates Force Transmission during Tissue Morphogenesis
复制标题
DOI：
10.1016/j.devcel.2016.11.003
复制
发表时间：
2016-12-05
影响因子：
11.8
通讯作者：
Tanentzapf, Guy
中科院分区：
文献类型：
--
作者：
Goodwin, Katharine;Ellis, Stephanie J.;Tanentzapf, Guy
组织形态发生需要力产生机制将细胞组织成复杂的结构。尽管许多此类机制已被表征，但我们对力如何在发育中的组织中整合知之甚少。我们提供的证据表明，整合素介导的细胞-细胞外基质（ECM）粘附调节果蝇背侧闭合（DC）过程中顶部产生的张力的传递。在羊膜浆膜 (AS) 的基底表面上发现了类似于粘着斑的含有整合素的粘附结构，羊膜浆膜是 DC 所必需的胚胎外上皮。整合素介导的粘附的基因调节导致有缺陷的 DC。定量图像分析和激光消融实验表明，基底细胞-ECM 粘附可抵抗顶端细胞位移和 AS 中相邻细胞之间的力传递。最后，我们提供了整合素依赖性力向 AS 基底传递的证据。总的来说，我们发现整合素调节细胞内和细胞间的力传递，从而在发育组织中传递张力方面发挥重要作用。
Tissue morphogenesis requires force-generating mechanisms to organize cells into complex structures. Although many such mechanisms have been characterized, we know little about how forces are integrated across developing tissues. We provide evidence that integrin-mediated cell-extracellular matrix (ECM) adhesion modulates the transmission of apically generated tension during dorsal closure (DC) in Drosophila. Integrin-containing adhesive structures resembling focal adhesions were identified on the basal surface of the amnioserosa (AS), an extraembryonic epithelium essential for DC. Genetic modulation of integrin-mediated adhesion results in defective DC. Quantitative image analysis and laser ablation experiments reveal that basal cell-ECM adhesions provide resistance to apical cell displacements and force transmission between neighboring cells in the AS. Finally, we provide evidence for integrin-dependent force transmission to the AS substrate. Overall, we find that integrins regulate force transmission within and between cells, thereby playing an essential role in transmitting tension in developing tissues.