Development of real-time radioisotope imaging systems for plant nutrient uptake studies
Development of real-time radioisotope imaging systems for plant nutrient uptake studies
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DOI：
10.1098/rstb.2011.0229
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发表时间：
2012-06-05
影响因子：
6.3
通讯作者：
Nakanishi, Tomoko M.
中科院分区：
文献类型：
--
作者：
Kanno, Satomi;Yamawaki, Masato;Nakanishi, Tomoko M.
离子营养对植物发育至关重要。已经开发了许多技术来成像和（或）测量植物中的离子运动。然而，它们大多是破坏性的，限制了分析。在这里，我们提出了放射性同位素成像技术的发展，克服了这些限制，并允许离子运动的实时成像。第一个系统，称为宏观成像，是为了可视化和测量整个植物尺度上器官之间的离子摄取和转运。这种装置与样品的照明完全兼容。我们还对荧光显微镜进行了改进，以建立不同的溶液，在微观水平上进行离子摄取分析。这两种系统都允许对图像进行数值分析，并具有广泛的动态检测范围，因为它们是基于放射性的。
Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive and limit the analysis. Here, we present the development of radioisotope imaging techniques that overcome such restrictions and allow for real-time imaging of ionic movement. The first system, called macroimaging, was developed to visualize and measure ion uptake and translocation between organs at a whole-plant scale. Such a device is fully compatible with illumination of the sample. We also modified fluorescent microscopes to set up various solutions for ion uptake analysis at the microscopic level. Both systems allow numerical analysis of images and possess a wide dynamic range of detection because they are based on radioactivity.