Macrophage migration inhibitory factor evaluation compared with prostate specific antigen as a biomarker in patients with prostate carcinoma
Macrophage migration inhibitory factor evaluation compared with prostate specific antigen as a biomarker in patients with prostate carcinoma
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DOI：
10.1002/cncr.10354
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发表时间：
2002-03-01
期刊：
影响因子：
6.2
通讯作者：
Tannenbaum, M
中科院分区：
文献类型：
--
作者：
Meyer-Siegler, KL;Bellino, MA;Tannenbaum, M
背景细胞因子是构成一类化学介体分子的多肽，其通过诱导特异性靶基因表达来调节细胞生长。本研究的目的是评估血清细胞因子巨噬细胞移动抑制因子（MIF）在接受常规前列腺特异性抗原（PSA）筛查的患者中的临床应用价值。在这项初步的回顾性研究中，作者报告了酶联免疫吸附试验（ELISA）用于血清样本中MIF测定的发展。聚合酶链反应（PCR）为基础的分析研究之间的关联MIF表达和前列腺癌（CaP）。作者开发了一种相对定量的逆转录酶-PCR检测方法，以确定激光捕获显微镜（LCM）解剖的前列腺上皮细胞中MIF mRNA的含量。血清MIF水平和总PSA水平的比较确定了正相关性（相关系数[r2] = 0.61; P < 0.001; n = 509例患者），表明这些蛋白质的血清浓度升高与CaP之间存在关联。血清MIF水平与CaP诊断的相关性表明，既往诊断为CaP的患者血清MIF浓度显著升高（平均标准差，6.8 +/- 0.87 ng/mL; P < 0.001）。为了将改变的血清MIF水平与前列腺上皮细胞内的MIF mRNA表达相关联，使用LCM解剖的前列腺上皮细胞（来自三个不同患者的福尔马林固定的活组织检查）通过PCR分析来确定MIF mRNA量。平均而言，在同一活检组织中，浸润到边缘的CaP上皮细胞中的MIF mRNA量是正常前列腺上皮细胞中的MIF mRNA量的6.5倍。本研究的ELISA数据提示MIF表达增加与CaP之间存在关联，并提示血清MIF浓度可作为CaP的预后标志物。(C)2002年美国癌症协会。
BACKGROUND. Cytokines are polypeptides that constitute a class of chemical mediator molecules that modulate cell growth by inducing specific target gene expression. The objective of this study was to evaluate the clinical usefulness of serum evaluation of the cytokine macrophage migration inhibitory factor (MIF) in patients undergoing routine prostate specific antigen (PSA) screening.METHODS. In this preliminary, retrospective study, the authors report the development of an enzyme-linked immunosorbent assay (ELISA) for MIF determination in serum samples. A polymerase chain reaction (PCR)-based assay investigated associations between MIF expression and prostate carcinoma (CaP). The authors developed a relative quantitative reverse transcriptase-PCR assay to determine MIF mRNA amounts within laser-capture microscopy (LCM)-dissected prostate epithelial cells.RESULTS. A comparison of serum MIF levels and total PSA levels identified a positive correlation (correlation coefficient [r2] = 0.61; P < 0.001; n = 509 patients), suggesting an association between elevated serum concentrations of these proteins and CaP. A correlation of serum MIF levels with a diagnosis of CaP demonstrated that patients with a previous CaP diagnosis had significantly elevated serum MIF concentrations (mean standard deviation, 6.8 +/- 0.87 ng/mL; P < 0.001). To associate altered serum MIF levels with MIF mRNA expression within prostate epithelial cells, LCM-dissected prostate epithelial cells (formalin fixed biopsies from three different patients) were used to determine MIF mRNA amounts by PCR analysis. On average, MIF mRNA amounts were 6.5 times higher in CaP epithelial cells that were invasive to the margin compared with MIF mRNA amounts in normal prostate epithelial cells within the same biopsy specimen.CONCLUSIONS. The ELISA data from the current study suggested an association between increased MIF expression and CaP and suggested that serum MIF concentration may serve as a prognostic marker for CaP. (C) 2002 American Cancer Society.