Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes
Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes
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DOI：
10.1016/j.bbrc.2007.06.166
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发表时间：
2007-09-14
影响因子：
3.1
通讯作者：
Johnson, Eric A.
中科院分区：
文献类型：
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作者：
Marshall, Kristin M.;Bradshaw, Marite;Johnson, Eric A.
肉毒杆菌是人类和动物的重要病原体，产生肉毒神经毒素（BoNT），这是已知的毒性最强的毒素。我们通过脉冲场凝胶电泳 (PFGE) 和 Southern 杂交确定 Loch Maree（A3 亚型）和 657Ba（B 型和 A4 亚型）菌株中编码 BoNT 的基因位于大（类似于 280 kb）质粒上。这是首次在肉毒杆菌A型和B型血清中证实质粒携带的神经毒素基因。在染色体外元件上发现A型和B型BoNT基因对于梭状芽胞杆菌神经毒性的进化，包括肉毒杆菌神经毒素基因的起源、表达和横向转移具有重要意义。 (c) 2007 Elsevier Inc. 保留所有权利。
Clostridium botulinum, an important pathogen of humans and animals, produces botulinum neurotoxin (BoNT), the most poisonous toxin known. We have determined by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations that the genes encoding BoNTs in strains Loch Maree (subtype A3) and 657Ba (type B and subtype A4) are located on large (similar to 280 kb) plasmids. This is the first demonstration of plasmid-borne neurotoxin genes in Clostridium botulinum serotypes A and B. The finding of BoNT type A and B genes on extrachromosomal elements has important implications for the evolution of neurotoxigenicity in clostridia including the origin, expression, and lateral transfer of botulinum neurotoxin genes. (c) 2007 Elsevier Inc. All rights reserved.