Consistent hepatic metastasis of human colorectal cancer in severe combined immunodeficient mice.
Consistent hepatic metastasis of human colorectal cancer in severe combined immunodeficient mice.
复制标题
严重联合免疫缺陷小鼠中人类结直肠癌的一致肝转移。
DOI：
10.1006/jsre.1996.0117
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Bankert,RB
中科院分区：
文献类型：
--
作者：
Bumpers,HL;Alosco,TR;Wang,HQ;Petrelli,NJ;Hoover,EL;Bankert,RB
人类结直肠癌转移研究的一个主要障碍是缺乏一种能够产生肝转移的有效体内模型。本研究将结直肠癌手术标本植入scid小鼠体内，研究其移植、生长和产生肝转移的能力。人类结直肠癌会在皮下和腹腔内植入和繁殖。54只皮下植入肿瘤的动物中有3只（6%）发生零星转移到肝脏。25只植入性腺脂肪垫的小鼠中有24只（96%）发生肝转移。肿瘤在皮下生长到非常大的体积并没有增加转移的可能性，在皮下空间的长期生长也没有增加转移的可能性。放置在性腺脂肪中的肿瘤不需要特殊的操作，在大多数情况下，植入的是一块实体瘤。在这种情况下，杂交证实了人类组织在这些转移性肿瘤中的持久性。我们的模型将允许研究涉及实体瘤转移的过程，原发肿瘤和转移瘤之间的差异特征，以及评估可能的治疗方式。
A major stumbling block in the study of human colorectal cancer metastasis has been the lack of an effectivein vivomodel producing liver metastasis on a consistent basis. In this study surgical specimens of colorectal carcinoma were implanted in scid mice and studied for engraftment, growth, and the capacity to produce hepatic metastases. Human colorectal cancers would engraft and propagate in the subcutis and intraperitoneally. Sporadic metastasis to the liver occurred in 3 of 54 (6%) animals with cancer implanted subcutaneously. Liver metastasis occurred in 24 of 25 (96%) mice with cancer implanted in the gonad fat pad. Tumor growth to extremely large volumes subcutaneously did not enhance metastatic potential, and neither did longer term growth in the subcutaneous space. Tumor placed in the gonad fat required no special manipulation and in most cases a single piece of solid tumor was implanted.In situhybridization confirmed the persistence of the human tissue in these metastasizing tumors. Our model will allow for the study of the processes involved in metastasis of solid tumors, characterization of differences between the primary tumor and the metastatic one, and evaluation of possible therapeutic modalities.