Weight gain in response to high-fat feeding in CD-1 male mice
Weight gain in response to high-fat feeding in CD-1 male mice
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DOI：
10.1258/la.2010.009114
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发表时间：
2010-07-01
影响因子：
2.4
通讯作者：
McFarlin, Brian K.
中科院分区：
文献类型：
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作者：
Breslin, W. L.;Strohacker, K.;McFarlin, Brian K.
本研究的目的是比较远交 CD-1 雄性小鼠在高脂肪喂养期间的体重增加和食物摄入量，同时考虑几种不同的实验设计。这项研究是使用来自三个独立实验的数据完成的，旨在解决不同的实验设计问题。实验 1 比较了分组饲养或单独饲养的小鼠。实验 2 比较了青春期小鼠和年轻成年小鼠。实验3检查了在饮食诱导体重增加之前接受过运动训练的小鼠。使用重复测量方差分析和线性回归来分析每个实验的数据。虽然住房和年龄对体重增加没有显着影响，但之前接受过运动训练的小鼠比久坐的小鼠消耗的卡路里明显更多，同时保持了相当的体重。我们使用实验 1 和 2 的数据生成了一个线性预测模型，该模型将使研究人员能够计算达到目标体重所需的高脂肪喂养周数。我们的主要发现描述了利用远交小鼠饮食诱导体重增加模型时与实验设计相关并影响实验设计的问题，并将为未来的研究人员提供指导。
The purpose of this study was to compare weight gain and food intake during high-fat feeding in outbred CD-1 male mice while considering several different experimental designs. This study was completed using data from three separate experiments and was designed to address different experimental design issues. Experiment 1 compared mice housed in groups or singly. Experiment 2 compared adolescent and young adult mice. Experiment 3 examined mice that had been previously exercise-trained prior to diet-induced weight gain. Data from each experiment were analysed using repeated measures analysis of variance and linear regression. While housing and age did not significantly affect weight gain, mice that were previously exercise-trained consumed significantly more kilocalories than sedentary mice while maintaining comparable body weights. We generated a linear prediction model using data from Experiments 1 and 2 that will allow investigators to calculate the weeks of high-fat feeding needed to reach a target body weight. Our key findings characterize the issues related to and affecting experimental design when utilizing an outbred mouse diet-induced weight gain model and will serve as a guide for future researchers.