Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture.
Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture.
复制标题
当肾上腺皮质细胞培养时，p21Sdi1 的表达增加。
DOI：
10.1006/excr.1995.1359
复制
发表时间：
1995
影响因子：
3.7
通讯作者：
Hornsby,PJ
中科院分区：
文献类型：
--
作者：
Yang,L;Didenko,VV;Noda,A;Bilyeu,TA;Darlington,DJ;Smith,JR;Hornsby,PJ
研究了细胞周期抑制剂 p21Sdi1/WAF1/Cip1 在分化细胞类型（肾上腺皮质细胞）中不同培养阶段（从肾上腺细胞制备到长期生长后衰老）的表达。在牛肾上腺皮质细胞中，SDI1 的表达在培养物中比在体内高得多。早在用于准备细胞培养的组织解离程序开始后 3 小时就观察到 SDI1 mRNA 的升高，并伴随着与抗 p21Sdi1 抗体反应的蛋白质水平的升高。然后在铺板和随后的培养物中长期生长期间维持这种表达水平。牛肾上腺皮质细胞的生长和静止可以通过在培养基中添加或去除 FGF 来调节。在这些细胞中，由于 FGF 剥夺导致的长期有丝分裂静止，SDI1 mRNA 并未增加。在培养的胎儿人肾上腺皮质细胞中，在培养物寿命的所有阶段也检测到了 SDI1 mRNA，包括从肾上腺分离细胞并接种培养物后 2 天。中年细胞的 SDI1 mRNA 高于衰老的人类成纤维细胞。培养中接近衰老的人肾上腺皮质细胞克隆具有比早期传代细胞更高的 SDI1 mRNA。因此，无论在体内还是体外，肾上腺皮质细胞中的 SDI1 表达与有丝分裂静止无关，但细胞的分离和培养对其表达产生强大的诱导影响。
Expression of the cell cycle inhibitor p21Sdi1/WAF1/Cip1was investigated in a differentiated cell type, the adrenocortical cell, at different stages of culture, from the preparation of cells from the adrenal gland to senescence after long-term growth. In bovine adrenocortical cells, expression of SDI1 was much higher in culture than in vivo. Elevation of SDI1 mRNA, accompanied by elevation of the level of a protein reacting with anti-p21Sdi1antibodies, was observed as early as 3 h after the start of the tissue dissociation procedure used to prepare cells for culture. This level of expression was then maintained during plating and subsequent long-term growth in culture. Growth and quiescence in bovine adrenocortical cells can be modulated by inclusion or removal of FGF from the culture medium. In these cells SDI1 mRNA was not increased by long-term mitotic quiescence resulting from FGF deprivation. In cultured fetal human adrenocortical cells, SDI1 mRNA was also detected at all stages of the culture life span, including 2 days after isolation of cells from the adrenal gland and plating in culture. Midlife-span cells had higher SDI1 mRNA than senescent human fibroblasts. Clones of human adrenocortical cells nearing senescence in culture had somewhat higher SDI1 mRNA than early passage cells. Thus, SDI1 expression in adrenocortical cells is not associated with mitotic quiescence either in vivo or in vitro, yet isolation of the cells and culturing them exerts a powerful inductive influence on its expression.