Sub-cellular accumulation of magnetic nanoparticles in breast tumors and metastases
Sub-cellular accumulation of magnetic nanoparticles in breast tumors and metastases
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DOI：
10.1016/j.biomaterials.2005.10.013
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Soboyejo, WO
中科院分区：
文献类型：
--
作者：
Zhou, JK;Leuschner, C;Soboyejo, WO
在这项研究中，研究了超顺磁性氧化铁纳米颗粒（SPION）在乳腺肿瘤和外周器官中的亚细胞积聚。MNP与促黄体激素释放激素（LHRH）结合，其受体由大多数类型的乳腺癌细胞表达。在将纳米颗粒注射到携带MDA-MB-435S.luc肿瘤的雌性裸鼠中后，处死小鼠以收集肿瘤和外周器官用于生物学和TEM分析。发现LHRH缀合的SPION（LHRH-SPION）在癌细胞中积累，主要在原发性肿瘤和转移性肺中，在那里它们聚集形成簇。相反，大部分未结合的SPION被收集在肝细胞中。结果表明，LHRH-SPION可用于靶向乳腺癌原发灶和肺转移灶的癌细胞。TEM也被证明是一种有用的工具，用于研究SPION在肿瘤和组织中的亚细胞分布。(C)2005爱思唯尔有限公司保留所有权利。
In this study, the sub-cellular accumulation of superparamagnetic iron oxide nanoparticles (SPIONs) in breast tumors and peripheral organs were investigated. MNPs were conjugated with luteinizing hormone releasing hormone (LHRH), whose receptors are expressed by most types of breast cancer cells. After the nanoparticles were injected into female nude mice bearing MDA-MB-435S.luc tumors, the mice were sacrificed to collect tumors and peripheral organs for biological and TEM analyses. LHRH conjugated SPIONs (LHRH- SPIONs) were found to accumulate in cancer cells, mainly in the primary tumors and the metastatic lungs, where they aggregated to form clusters. In contrast, most of the unconjugated SPIONs were collected in the liver cells. The results suggest that LHRH- SPIONs can be used to target cancer cells in the primary breast tumors and the lung metastases. TEM is also shown to be a useful tool for the studies of sub-cellular distributions of SPIONs in tumors and tissues. (C) 2005 Elsevier Ltd. All rights reserved.