Domains of gene silencing near the left end of chromosome III in Saccharomyces cerevisiae.
Domains of gene silencing near the left end of chromosome III in Saccharomyces cerevisiae.
复制标题
酿酒酵母 III 号染色体左端附近的基因沉默域。
DOI：
10.1093/genetics/160.4.1401
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Bi,Xin
中科院分区：
文献类型：
--
作者：
Bi,Xin
在酿酒酵母中，端粒位点和端粒附近的区域在转录上是沉默的。HMLis位于3号染色体左端粒11kb处。我系统地研究了这个11kb染色体区域的基因沉默。我发现沉默至少延伸1.1 kb超出dhml，表明hmle消声器在两侧都起作用。此外，我获得的证据表明，E沉默者附近的0.71 kb序列对沉默传播起屏障作用，并且与silentHMLdomain的左边界一致。我还表明，端粒的沉默仅限于~ 2kb结构域。另一方面，hmlor与端粒之间约7kb的区域未被hmlor沉默。这些结果提供了真核生物基因组组织成具有不同基因表达潜力的分散结构域的清晰例子。
InSaccharomyces cerevisiaetheHMloci and regions adjacent to the telomeres are transcriptionally silent.HMLis situated 11 kb from the left telomere of chromosome III. I have systematically examined gene silencing along this 11-kb chromosomal region. I found that silencing extends at least 1.1 kb beyondHML, indicating that theHMLE silencer acts on both sides. Moreover, I obtained evidence indicating that a 0.71-kb sequence near the E silencer acts as a barrier to the spread of silencing and coincides with the left boundary of the silentHMLdomain. I also showed that silencing at the telomere is limited to an ~2-kb domain. On the other hand, an ~7-kb region betweenHMLand the telomere is not silenced byHMLor the telomere. These results provide a clear example of organization of the eukaryotic genome into interspersed domains with distinct potentials for gene expression.