IN VITRO ANTI-INFLAMMATORY MECHANISM OF FOLIUM HIBISCI MUTABILIS LEAVES ETHANOL EXTRACTS
IN VITRO ANTI-INFLAMMATORY MECHANISM OF FOLIUM HIBISCI MUTABILIS LEAVES ETHANOL EXTRACTS
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DOI：
10.4314/ajtcam.v11i1.19
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Wang, Xueyan
中科院分区：
文献类型：
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作者：
Wang, Jiangning;Li, Xiaorong;Wang, Xueyan
【摘要】：背景:木槿叶是具有愈合伤口、抗菌、抗炎等药理活性的中药之一,临床常用于治疗局部化脓性感染、烫伤、流行性腮腺炎等。但提取物对炎症大鼠RAW264.7细胞及血清TNF-α、IL-6、NO水平的影响却鲜有报道。 材料与方法：我们主要采用脂多糖(LPS)干预的巨噬细胞系RAW264.7建立炎症细胞模型。采用ELISA法检测FHMAEs作用下模型细胞中TNF、IL-6分泌水平的变化，硝酸还原酶法测定NO水平的变化；选择II型胶原诱导的关节炎模型，观察FHMAEs对血清IL-6和NO水平的调节作用，探讨FHMAEs治疗类风湿关节炎的作用机制。记录模型大鼠的状态。结果：LPS诱导的RAW264.7细胞中TNF-α、IL-6、NO的释放均受到显着抑制，且具有明确的剂量反应关系； FHMAEs降低了实验性关节炎大鼠血清中TNF-α、IL-6和NO的水平，改善了大鼠的状态。最后我们发现FHMAEs可大大降低炎症细胞中IL-6、NO和TNF-α的水平。结论：FHMAEs可减轻或抑制滑膜炎症和滑膜增殖，这可能为类风湿性关节炎的新药开发和临床治疗提供实验有效数据。
Background: Folium Hibisci Mutabilis was one of the traditional Chinese medicines with pharmacological activities of wound healing, antibacterial and anti-inflammatory, normally applied to the clinical treatment of local purulent infection, scald, and epidemic parotitis. However, few people reported that its effects of extracts on RAW264.7, cells and TNF-alpha, IL-6, and NO levels in rats serum of inflammation rats.Materials and Methods: We mainly adopted the RAW264.7, macrophage cell line which was intervened by lipopolysaccharide (L PS), to establish the model of inflammatory cells. The secretion changes of TNF, and IL-6, level in the model cell acted by the FHMAEs, were detected by ELISA, NO level changes were determined by nitrate reductase method; Arthritis model induced by Collagen Type II were chose, and regulatory role of FHMAEs on IL-6 and NO level in serum were observed, FHMAEs mechanism of action were investigated in the treatment of rheumatoid arthritis. The states of the model rats were recorded.Results: Release of TNF-alpha, IL-6, and NO in LPS-induced RAW264.7, cells were significantly inhibited and has a clear dose-response relationship; The TNF-alpha, IL-6 and NO levels were reduced by FHMAEs in experimental arthritis rats serum, and the status of rats were improved. At last, we found that IL-6, NO and TNF-alpha levels in the inflammatory cells could be reduced by FHMAEs greatly.Conclusion: Both synovial inflammation and synovial proliferation could be reduced or inhibited by FHMAEs, all of this may provide experimental effective data for the novel drugs development and the clinical treatment of rheumatoid arthritis.