The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing
The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing
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DOI：
10.1073/pnas.93.8.3602
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发表时间：
1996-04-16
影响因子：
11.1
通讯作者：
Nichol, ST
中科院分区：
文献类型：
--
作者：
Sanchez, A;Trappier, SG;Nichol, ST
1994年底和1995年初，埃博拉病毒在非洲再次出现，在象牙海岸和扎伊尔造成人类疾病。对这些病毒和其他EBO病毒亚型的完整糖蛋白基因的分析表明，病毒体糖蛋白（130 kDa）在两个阅读框中编码，这两个阅读框通过转录编辑连接。这种编辑导致在编码区中间附近的七个腺苷中添加额外的非模板化腺苷。主要基因产物是一种较小的（50-70 kDa）非结构性分泌糖蛋白，大量产生，功能未知。系统发育分析表明，EBO病毒亚型在遗传上是多样的，最近象牙海岸分离株代表了EBO病毒的一种新的（第四）亚型，相比之下，1995年在扎伊尔基奎特爆发的EBO病毒分离株与近20年前在扎伊尔扬布库引起类似流行病的病毒几乎相同。这种遗传稳定性可能表明EBO病毒与其天然宿主共同进化，并且在野外不会发生明显变化。
In late 1994 and early 1995, Ebola (EBO) virus dramatically reemerged in Africa, causing human disease in the Ivory Coast and Zaire. Analysis of the entire glycoprotein genes of these viruses and those of other EBO virus subtypes has shown that the virion glycoprotein (130 kDa) is encoded in two reading frames, which are linked by transcriptional editing. This editing results in the addition of an extra nontemplated adenosine within a run of seven adenosines near the middle of the coding region. The primary gene product is a smaller (50-70 kDa), nonstructural, secreted glycoprotein, which is produced in large amounts and has an unknown function. Phylogenetic analysis indicates that EBO virus subtypes are genetically diverse and that the recent Ivory Coast isolate represents a new (fourth) subtype of EBO virus, In contrast, the EBO virus isolate from the 1995 outbreak in Kikwit, Zaire, is virtually identical to the virus that caused a similar epidemic in Yambuku, Zaire, almost 20 years earlier. This genetic stability may indicate that EBO viruses have coevolved with their natural reservoirs and do not change appreciably in the wild.