Aquaporins 1, 3 and 8 expression and cytokines in irritable bowel syndrome rats' colon via cAMP-PKA pathway.
Aquaporins 1, 3 and 8 expression and cytokines in irritable bowel syndrome rats' colon via cAMP-PKA pathway.
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DOI：
10.2139/ssrn.3228209
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发表时间：
2018
影响因子：
1.4
通讯作者：
Guanqun Chao;Shuo Zhang
中科院分区：
文献类型：
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作者：
Guanqun Chao;Shuo Zhang
背景肠易激综合征（IBS）是最常见的胃肠道疾病之一。cAMP-PKA通路在介导细胞对各种刺激的反应中起关键作用，这两者在IBS中可能起重要作用。目的通过cAMP-PKA通路探讨IBS大鼠模型的发病机制，为进一步研究IBS的发病机制奠定基础。方法采用应激和条件刺激建立IBS大鼠模型。实验分为对照组、模型组和毛喉素组。免疫组化法检测大鼠结肠AQP 1、AQP 3、AQP 8和CREB（Ser 133）的表达。采用PCR技术检测结肠组织中AQP 1、AQP 3、AQP 8、IL-1β、TGF β、TNF α的表达。结果成功建立IBS大鼠模型。IBS大鼠结肠AQP 1、AQP 3、AQP 8和CREB（Ser 133）表达下调，AMP激活剂干预后表达上调（P<0.05）。肠易激综合征大鼠结肠组织中IL-1β、TGF β和TNF α的表达上调，AMP激活剂干预后表达下调（P<0.05）。结论炎症细胞因子的释放、免疫系统的激活和液态水代谢异常是IBS通过cAMP PKA途径发生的机制。
BACKGROUND Irritable bowel syndrome (IBS) is one of the most common gastrointestinal (GI) disorders. The cAMP-PKA pathway plays a key role in mediating cell responses to various stimuli both of which might play an important role in IBS. OBJECTIVE Our research aimed to detect the mechanism of IBS rat models via the cAMP-PKA pathway so as to further detect the mechanisms of IBS. METHODS IBS rats were built by stress and conditioned stimulus. The study was divided into 3 groups (a control group, a model group, and a forskolin group). The expressions of AQP1, AQP3, AQP8, and CREB (Serl33) in the colons of rats were detected by immunohistochemistry. The expressions of AQP1, AQP3, AQP8, IL-1β, TGF β, and TNF α in the colon were detected by the PCR technique. RESULTS The IBS rats were built successfully. The expressions of AQP1, AQP3, AQP8, and CREB (Serl33) were down-regulated in the colons of IBS rats and were up-regulated with the intervention of the activator of AMP (P<0.05). The expressions of IL-1β, TGF β and TNF α were up-regulated in the colons of IBS rats and were down-regulated with the intervention of the activator of AMP (P<0.05). CONCLUSIONS The release of inflammatory cytokines, the activation of the immune system and liquid water metabolic abnormalities are the mechanisms of IBS via the cAMP-PKA pathway.