Influenza A virus NS1 protein does not suppress RNA interference in mammalian cells
Influenza A virus NS1 protein does not suppress RNA interference in mammalian cells
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DOI：
10.1099/vir.0.81764-0
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发表时间：
2006-09-01
影响因子：
3.8
通讯作者：
Jin, Dong-Yan
中科院分区：
文献类型：
--
作者：
Kok, Kin Hang;Jin, Dong-Yan
甲型流感病毒 NS1 蛋白已被证明可以抑制植物和果蝇中的 RNA 干扰 (RNAi)。尽管尚未通过实验证明，NS1 也被认为可以抑制哺乳动物中的 RNAi，并且是干扰素的拮抗剂。本研究探讨了流感病毒A/WSN/33株NS1蛋白对哺乳动物细胞RNAi的影响。虽然瞬时或稳定表达的 NS1 完全能够抑制培养细胞中的干扰素途径，但它不会抑制 RNAi 介导的不同报告基因的沉默。这些发现意味着哺乳动物和植物之间的 RNAi 机制存在显着差异。
Influenza A virus NS1 protein has been shown to suppress RNA interference (RNAi) in plants and Drosophila. Although it has not been demonstrated experimentally, NS1 has also been thought to inhibit RNAi in mammals as well as being an antagonist of interferon. In this study, the influence of NS1 protein from influenza virus strain A/WSN/33 on RNAi in mammalian cells was investigated. While transiently or stably expressed NS1 was fully competent to inhibit the interferon pathway in cultured cells, it did not suppress RNAi-mediated silencing of different reporter genes. These findings imply a significant difference in RNAi mechanism between mammals and plants.