Nematode-specific PCR primers for the 18S small subunit rRNA gene
Nematode-specific PCR primers for the 18S small subunit rRNA gene
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DOI：
10.1111/j.1471-8286.2005.01009.x
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发表时间：
2005-09-01
期刊：
影响因子：
--
通讯作者：
Smith, CR
中科院分区：
文献类型：
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作者：
Floyd, RM;Rogers, AD;Smith, CR
设计了一组聚合酶链式反应引物，可扩增 18S 核糖体 DNA 基因的 c.1 kb 片段，并且对线虫门具有特异性。事实证明，这些技术可用于从与其他生物材料混合的样品中分离线虫基因，特别是在 DNA 条形码应用中。描述了最佳反应条件。这些引物已成功从广泛的线虫系统发育范围中扩增出正确的片段，并且迄今为止尚未产生任何其他生物群的序列。
A set of polymerase chain reaction primers were designed, which amplify a c.1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.