Silver nanoparticle-embedded hydrogel as a photothermal platform for combating bacterial infections
Silver nanoparticle-embedded hydrogel as a photothermal platform for combating bacterial infections
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嵌入银纳米颗粒的水凝胶作为对抗细菌感染的光热平台
DOI：
10.1016/j.cej.2019.122990
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发表时间：
2020-02-15
影响因子：
15.1
通讯作者：
Wang, Jihao
中科院分区：
文献类型：
--
作者：
Liu, Yingnan;Li, Fan;Wang, Jihao
由于抗生素的滥用和耐药性的威胁，细菌性伤口感染仍然是最棘手的医学问题之一。因此，开发新型抗菌平台具有重要意义。采用一锅法成功制备了没食子酸功能化银纳米粒子（GA-Ag NPs），并将其嵌入到生物相容性多糖的网络结构中，得到了一种新型的具有良好杀菌效果的水凝胶（GA-Ag NP水凝胶）。在该系统中，包埋的GA-Ag纳米粒子不仅作为抗菌剂从水凝胶基质中持续释放以实现杀菌，而且作为光热剂有效且可控地将808 nm的近红外线光转化为热量以杀灭细菌。体外试验证实GA-Ag NP水凝胶对革兰氏阴性大肠杆菌（E.大肠杆菌）和革兰氏阳性金黄色葡萄球菌（S. aureus)具有良好的抗菌活性。体内伤口愈合实验证实了这种水凝胶在对抗细菌感染和加速伤口愈合方面的实际潜力。溶血和细胞毒性实验表明，其具有良好的生物相容性。这种新型抗菌水凝胶的开发为加强抗细菌感染的抗菌剂库开辟了新的途径。
Because of the indiscriminate use of antibiotics and threats of the drug resistance, bacterial wound infection remains one of the most difficult medical issues. Therefore, it is of great significance to develop novel antibacterial platforms. In this work, we successfully prepared gallic acid functional silver nanoparticles (GA-Ag NPs) by a one-pot method and then embedded them into the network structure of a biocompatible polysaccharide to obtain a novel hydrogel (GA-Ag NP hydrogel) with outstanding bactericidal effect. In this system, the embedded GA-Ag NPs not only act as an antibacterial agent sustainably released from the matrix of the hydrogel to realize sterilization, but also act as a photothermal agent that effectively and controllably converts 808 nm near-infrared ray light into heat to kill bacteria. In vitro testing verified the outstanding broad-spectrum sterilization property of the GA-Ag NP hydrogel against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). In vivo wound healing experiments confirmed the practical potential of this hydrogel in combating bacterial infections and accelerating wound healing. Hemolysis and cytotoxicity experiments showed that it has good biocompatibility. The development of this novel antibacterial hydrogel opens a new way to strengthen the antimicrobial arsenal for fighting bacterial infections.