Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity
Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity
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DOI：
10.1111/mec.12183
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发表时间：
2013-02-01
影响因子：
4.9
通讯作者：
Leendertz, Fabian H.
中科院分区：
文献类型：
--
作者：
Calvignac-Spencer, Sebastien;Merkel, Kevin;Leendertz, Fabian H.
大规模监测计划对于评估全球哺乳动物生物多样性至关重要，在这一框架内，水蛭最近被宣传为陆地哺乳动物物种DNA的间接来源。食腐肉的苍蝇无处不在，可以预期以许多脊椎动物的尸体为食。因此，我们测试了苍蝇衍生的DNA分析是否也可以作为一种新的工具，哺乳动物多样性调查。我们筛选了从201腐尸苍蝇收集在热带栖息地的科特迪瓦和马达加斯加的哺乳动物DNA提取的DNA，使用多重PCR系统和检索的DNA序列从一组不同的物种（22在科特迪瓦，4在马达加斯加），利用不同的森林地层，并显示了广泛的身体大小。从蝇池产生的扩增子的深度测序与单独测序方法同样好地进行。我们的结论是，苍蝇衍生的DNA的分析可以实施一个非常快速和具有成本效益的方式，并会给当地哺乳动物的多样性相对公正的图片。因此，腐尸蝇代表了一种非凡的、迄今为止尚未开发的哺乳动物DNA资源，这可能会被证明对未来野生哺乳动物群落的清单有用。
Large-scale monitoring schemes are essential in assessing global mammalian biodiversity, and in this framework, leeches have recently been promoted as an indirect source of DNA from terrestrial mammal species. Carrion feeding flies are ubiquitous and can be expected to feed on many vertebrate carcasses. Hence, we tested whether fly-derived DNA analysis may also serve as a novel tool for mammalian diversity surveys. We screened DNA extracted from 201 carrion flies collected in tropical habitats of Cote d'Ivoire and Madagascar for mammal DNA using multiple PCR systems and retrieved DNA sequences from a diverse set of species (22 in Cote d'Ivoire, four in Madagascar) exploiting distinct forest strata and displaying a broad range of body sizes. Deep sequencing of amplicons generated from pools of flies performed equally well as individual sequencing approaches. We conclude that the analysis of fly-derived DNA can be implemented in a very rapid and cost-effective manner and will give a relatively unbiased picture of local mammal diversity. Carrion flies therefore represent an extraordinary and thus far unexploited resource of mammal DNA, which will probably prove useful for future inventories of wild mammal communities.