CD8+ lymphocytes from patients with systemic lupus erythematosus sustain, rather than suppress, spontaneous polyclonal IgG production and synergize with CD4+ cells to support autoantibody synthesis.
CD8+ lymphocytes from patients with systemic lupus erythematosus sustain, rather than suppress, spontaneous polyclonal IgG production and synergize with CD4+ cells to support autoantibody synthesis.
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系统性红斑狼疮患者的 CD8 淋巴细胞维持而不是抑制自发多克隆 IgG 的产生，并与 CD4 细胞协同支持自身抗体的合成。
DOI：
10.1002/art.1780330823
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发表时间：
1990
影响因子：
--
通讯作者：
Horwitz,DA
中科院分区：
文献类型：
--
作者：
Linker-Israeli,M;QuismorioJr,FP;Horwitz,DA
探讨系统性红斑狼疮(SLE)B细胞高活性的细胞需求。CD8+或CD4+淋巴细胞的去除显著降低了SLE外周血单核细胞(PBMC)体外自发产生的多克隆抗体和抗原特异性抗体(抗双链DNA和抗核蛋白)。持续产生免疫球蛋白的CD8+淋巴细胞为CD3+、人类白细胞抗原DR+，经抗人类白细胞抗原DR单抗预先孵育后，其活性被抑制。当CD4+和CD8+细胞同时被去除时，两个亚群中的任何一个亚群的重现部分恢复了多克隆抗体的产生，但这两个细胞亚群都需要重建自身抗体的产生。纯化的SLE B细胞仅产生未分离的PBMC产生的15%的IgG，仅由CD4+与CD8+细胞以及与CD8-、CD4-、CD16+细胞的混合而完全重组。至少部分支持自发产生免疫球蛋白的因素可以归因于内源性白介素2和白介素6。
The cellular requirements for B cell hyperactivity in systemic lupus erythematosus (SLE) were studied. Removal of either CD8+ or CD4+ lymphocytes markedly decreased the spontaneous in vitro production of polyclonal IgG and of antigen‐specific (anti‐double‐stranded DNA and antinucleoprotein) antibodies by SLE peripheral blood mononuclear cells (PBMC). The CD8+ lymphocytes that sustained IgG production were CD3+, HLA‐DR+, and their activity was abrogated by preincubation with anti‐HLA‐DR monoclonal antibody. When both CD4+ and CD8+ cells were removed, the readdition of either subset partially restored polyclonal IgG production, but both cell subsets were required to reconstitute autoantibody production. Purified SLE B cell cultures, which generated only 15% of the IgG produced by unseparated PBMC, were fully reconstituted only by mixtures of CD4+ with CD8+ cells, and with CD8‐, CD4‐, CD16+ cells. At least part of the support for spontaneous IgG production can be attributed to endogenous interleukin‐2 and interleukin‐6.