Microengineering the environment of mammalian cells in culture
Microengineering the environment of mammalian cells in culture
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DOI：
10.1557/mrs2005.52
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发表时间：
2005-03-01
期刊：
影响因子：
5
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Chen, CS;Jiang, XY;Whitesides, GM
基于观察单个细胞对其环境的生物学反应的分析有可能对细胞生物学和生物医学做出巨大贡献。为了用细胞进行明确的实验，细胞生存的环境和细胞本身都必须明确。基于细胞的测定现在受到不一致性和不可再现性的困扰，并且信息测定开发中的主要挑战是理解这些不一致性的基本基础并限制它们。现在看来，多种因素可能导致单个细胞对刺激反应的变异性;其中一些因素可能是细胞外在的，一些因素可能是内在的。基于微工程的新技术，特别是使用软光刻技术在分子水平上形成表面图案和制造微流体系统，为解决外在因素提供了新的能力。这篇综述讨论了材料科学的最新进展，这些进展提供了定义明确的物理环境，可用于研究细胞，无论是单独的还是成组的，在附着培养中。它还审查了必须解决的挑战，以使基于细胞的测定重现。
Assays based on observations of the biological responses of individual cells to their environment have the potential to make enormous contributions to cell biology and biomedicine. To carry out well-defined experiments using cells, both the environments in which the cells live and the cells themselves must be well defined. Cell-based assays are now plagued by inconsistencies and irreproducibility, and a primary challenge in the development of informative assays is to understand the fundamental bases for these inconsistencies and to limit them. It now seems that multiple factors may contribute to the variability in the response of individual cells to stimuli; some of these factors may be extrinsic to the cells, some intrinsic. New techniques based on microengineering especially using soft lithography to pattern surfaces at the molecular level and to fabricate microfluidic systems-have provided new capabilities to address the extrinsic factors. This review discusses recent advances in materials science that provide well-defined physical environments that can be used to study cells, both individually and in groups, in attached culture. It also reviews the challenges that must be addressed in order to make cell-based assays reproducible.