A DNA-based method for rationally assembling nanoparticles into macroscopic materials
A DNA-based method for rationally assembling nanoparticles into macroscopic materials
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DOI：
10.1038/382607a0
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发表时间：
1996-08-15
期刊：
影响因子：
64.8
通讯作者：
Storhoff, JJ
中科院分区：
文献类型：
--
作者：
Mirkin, CA;Letsinger, RL;Storhoff, JJ
胶体，金属和半导体的颗粒具有潜在的有用的光学，光电和材料特性（1-4），这些特性源于它们的小（纳米级）尺寸。这些性质可能导致包括化学传感器、光谱增强剂、量子点和纳米结构制造以及显微成像方法在内的应用（2-4）。现在可以对胶体颗粒的化学组成，尺寸和多分散性进行大量控制，并且已经开发了许多方法将它们组装成有用的聚集体和材料，在这里，我们描述了一种将胶体金纳米颗粒合理且可逆地组装成宏观聚集体的方法。该方法涉及将两批13 nm金颗粒的表面连接到与金结合的巯基封端的非互补DNA寡核苷酸，当我们向溶液中添加具有与两个接枝序列互补的“粘性末端”的寡核苷酸双链体时，纳米颗粒自组装成聚集体，该组装过程可以通过热变性逆转。这种策略现在应该可以通过使用DNA相互作用的特异性来指导不同大小和组成的颗粒之间的相互作用，从而定制胶体聚集体的光学，电子和结构特性。
COLLOIDAL, particles of metals and semiconductors have potentially useful optical, optoelectronic and material properties(1-4) that derive from their small (nanoscopic) size. These properties might lead to applications including chemical sensors, spectroscopic enhancers, quantum dot and nanostructure fabrication, and microimaging methods(2-4). A great deal of control can now be exercised over the chemical composition, size and polydispersity(1,2) of colloidal particles, and many methods have been developed for assembling them into useful aggregates and materials, Here we describe a method for assembling colloidal gold nanoparticles rationally and reversibly into macroscopic aggregates. The method involves attaching to the surfaces of two batches of 13-nm gold particles non-complementary DNA oligonucleotides capped with thiol groups, which bind to gold, When we add to the solution an oligonucleotide duplex with 'sticky ends' that are complementary to the two grafted sequences, the nanoparticles self-assemble into aggregates, This assembly process can be reversed by thermal denaturation. This strategy should now make it possible to tailor the optical, electronic and structural properties of the colloidal aggregates by using the specificity of DNA interactions to direct the interactions between particles of different size and composition.