Rebound tonometry in conscious, conditioned mice avoids the acute and profound effects of anesthesia on intraocular pressure
Rebound tonometry in conscious, conditioned mice avoids the acute and profound effects of anesthesia on intraocular pressure
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DOI：
10.1089/jop.2007.0114
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发表时间：
2008-04-01
影响因子：
2.3
通讯作者：
Toris, Carol B.
中科院分区：
文献类型：
--
作者：
Johnson, Thomas V.;Fan, Shan;Toris, Carol B.
目的：本研究的目的是评估在清醒的条件反射小鼠中通过市售回弹眼压计进行多次眼内压（IOP）测量的准确性、可重复性和安全性，并表征麻醉对小鼠IOP的急性和深刻影响。为了测试眼压计的准确性，在氯胺酮/甲苯噻嗪麻醉下的CD-1小鼠的IOP在实验上设定，并在经角膜插管后用水压计/换能器系统监测，同时进行眼压测量。在清醒、受约束的小鼠中测试眼压计的长期和短期可重复性，因为连续4天每天下午测量一次。在第5天，在相同的小鼠中每4分钟测量一次IOP，持续32分钟。在2个单独的日子，在交叉设计中，对小鼠施用氯胺酮/甲苯噻嗪或2，2，2-三溴乙醇麻醉，每2 min测眼压1次，共32 min。清醒小鼠在滴入马来酸噻吗洛尔前和滴入后1 h进行反跳眼压测量结果：在8 ~ 38 mmHg压力范围内，眼压测量与眼压测量相关性良好（y = 0.98x - 0.32，R-2 = 0.94; P < 0.001）。平均眼压测量IOP在4天内保持不变（范围，11.7-13.2 mmHg）。IOPS显著下降（P
Aims: The aims of this study were to evaluate the accuracy, repeatability, and safety of multiple intraocular pressure (IOP) measurements by a commercially available rebound tonometer in conscious, conditioned mice, and to characterize the acute and profound effects of anesthesia on IOP in mice.Methods: To test the accuracy of the tonometer, IOPs of CD-1 mice under ketamine/xylazine anesthesia were experimentally set and monitored with a water manometer/transducer system following transcorneal cannulation while simultaneously performing tonometry. The long- and short-term repeatability of the tonometer was tested in conscious, restrained mice, as measurements were taken once-daily in the afternoon for 4 consecutive days. On day 5, IOPs were measured in the same mice once every 4 min for 32 min. On 2 separate days, mice were administered ketamine/xylazine or 2,2,2-tribromoethanol anesthesia, in a crossover design, and IOPs were measured once every 2 min for 32 min. Rebound tonometry was performed in conscious mice before and 1 hour after 1 drop of timolol maleate (10 mu L of 0.5%) application to 1 eye.Results: IOP measurements by rebound tonometry correlated well with manometry for pressures between 8 and 38 mmHg (y = 0.98x - 0.32, R-2 = 0.94; P < 0.001). The, average tonometric IOP was invariant over 4 days (range, 11.7-13.2 mmHg). IOPs dropped significantly (P