The mitotic apparatus: isolation by controlled pH.
The mitotic apparatus: isolation by controlled pH.
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DOI：
10.1083/jcb.12.1.47
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发表时间：
1962-01
期刊：
影响因子：
--
通讯作者：
KANE RE
中科院分区：
文献类型：
--
作者：
KANE RE
对从棘皮动物卵中分离有丝分裂器（MA）进行了详细研究，特别关注控制其稳定性的因素。成功的分离主要取决于分离溶液的pH值，需要轻微的酸值。使用 1 M 己二醇溶液（pH 6.0 至 6.4 缓冲）可得到稳定 MA 的高产率，而质量较差的 MA 可以在 pH 5.5 至 5.8 缓冲的水中分离。分离只能在非常窄的 pH 范围内进行，因为在较低的 pH 值下，细胞更难以破裂，而在较高的 pH 值下，MA 会变得不稳定。在此范围内，MA 的纤维结构随 pH 值变化。当转移到 pH 7 的水中时，分离的 MA 会缓慢崩解，并在高离子强度的溶液中快速溶解。
The isolation of the mitotic apparatus (MA) from the echinoderm egg was studied in detail, with particular attention given to the factors governing its stability. Successful isolation depends mainly on the pH of the isolation solution, slightly acid values being required. The use of a 1 M solution of hexanediol, buffered at pH 6.0 to 6.4, gives high yields of stable MA, while MA of poorer quality can be isolated in water buffered at pH 5.5 to 5.8. Isolation is possible only over a very narrow range of pH, as the cells become more difficult to break at lower values and the MA becomes unstable at higher values. Within this range the fibrous structure of the MA varies with the pH. The isolated MA disintegrates slowly when transferred to water at pH 7 and dissolves rapidly in solutions of high ionic strength.