PHOSPHORYLATION OF THE LYMPHOID-CELL KINASE P56LCK IS STIMULATED BY MICROMOLAR CONCENTRATIONS OF ZN-2+
PHOSPHORYLATION OF THE LYMPHOID-CELL KINASE P56LCK IS STIMULATED BY MICROMOLAR CONCENTRATIONS OF ZN-2+
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DOI：
10.1016/0014-5793(91)80411-u
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发表时间：
1991-04-09
期刊：
影响因子：
3.5
通讯作者：
GACON, G
中科院分区：
文献类型：
--
作者：
PERNELLE, JJ;CREUZET, C;GACON, G
在 LSTRA 淋巴瘤细胞的颗粒部分中，在不存在其他二价阳离子的情况下，Zn2+ 会引发淋巴特异性酪氨酸激酶 p56lck 的酪氨酸磷酸化。 单独的 Zn2+ 也能诱导免疫沉淀 p56lck 的自磷酸化。 Zn2+ 的作用具有剂量依赖性；它在 Zn2+ 浓度低至 5-mu-M 时即可检测到，并在 100-mu-M Zn2+ 时达到最大值。 除其他测试的二价阳离子外。 Mn2+ 和较小程度的 Co2+ 也有效。 在生理浓度的 Mg2+ 离子存在下，Zn2+ 也会刺激 p56lck 磷酸化，而原钒酸盐则没有影响。 这些结果表明 Zn2+ 激活 p56lck 的自磷酸化；这可能与Zn2+对T淋巴细胞活化的刺激作用有关。
In particulate fractions from LSTRA lymphoma cells, tyrosine phosphorylation of the lymphoid specific tyrosine kinase p56lck is elicited by Zn2+ in the absence of other divalent cations. Zn2+ alone also induces autophosphorylation of immunoprecipitated p56lck. The effect of Zn2+ is dose dependent; it is detected at concentrations of Zn2+ as low as 5-mu-M and reaches a maximum at 100-mu-M Zn2+. Among other divalent cations tested. Mn2+, and Co2+ to a lesser extent, were also effective. Zn2+ also stimulated p56lck phosphorylation in the presence of Mg2+ ions at physiological concentration, whereas orthovanadate had no effect. These results suggest that Zn2+ activates the autophosphorylation of p56lck; this fact could be related with the stimulating effect of Zn2+ in the activation of T lymphocytes.