Albumin nanoparticles with synergistic antitumor efficacy against metastatic lung cancers
Albumin nanoparticles with synergistic antitumor efficacy against metastatic lung cancers
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DOI：
10.1016/j.colsurfb.2017.06.039
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发表时间：
2017-10-01
影响因子：
5.8
通讯作者：
Youn, Yu Seok
中科院分区：
文献类型：
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作者：
Kim, Bomi;Seo, Bohyung;Youn, Yu Seok
白蛋白纳米粒是众所周知的用于递送疏水性化疗剂的有效药物载体。使用稍微修改的纳米颗粒白蛋白结合（nab（TM））技术制备了包裹姜黄素和阿霉素的白蛋白纳米颗粒，并检查了这两种药物的协同作用。使用高压均质器制备包封姜黄素、多柔比星以及姜黄素和多柔比星两者的白蛋白纳米颗粒。白蛋白纳米粒的大小与130 nm相似，这被认为适合于EPR（增强的渗透性和保留）效果。白蛋白纳米粒在24 h内逐渐释放药物，无突释效应。为了证实两种药物的协同作用，使用B16 F10黑素瘤细胞进行体外细胞毒性测定。与姜黄素和多柔比星单独处理相比，姜黄素和多柔比星共同处理对B16 F10黑色素瘤细胞的细胞毒性作用最高。用中效方程计算的综合指数为0.6069，表明有增效作用。共聚焦激光扫描显微镜和荧光激活细胞分选的结果对应于体外细胞毒性试验的结果，表明两种药物诱导的协同细胞毒性。使用由B16 F10肺转移诱导的C57 BL/6小鼠模型来研究体内治疗效果。当姜黄素和多柔比星同时治疗时，与单独使用姜黄素或多柔比星相比，肺中的转移性黑色素瘤肿块肉眼可见地减少。包封两种抗癌药物的白蛋白纳米颗粒显示出具有有效的治疗结果，并且将是治疗耐药肺癌的极好方法。(C)2017爱思唯尔B. V.保留所有权利。
Albumin nanoparticles are well-known as effective drug carriers used to deliver hydrophobic chemotherapeutic agents. Albumin nanoparticles encapsulating curcumin and doxorubicin were fabricated using slightly modified nanoparticle albumin-bound (nab (TM)) technology, and the synergistic effects of these two drugs were examined. Albumin nanoparticles encapsulating curcumin, doxorubicin, and both curcumin and doxorubicin were prepared using a high pressure homogenizer. The sizes of albumin nanoparticles were similar to 130 nm, which was considered to be suitable for the EPR (enhanced permeability and retention) effect. Albumin nanoparticles gradually released drugs over a period of 24 h without burst effect. To confirm the synergistic effect of two drugs, in vitro cytotoxicity assay was performed using B16F10 melanoma cells. The cytotoxic effect on B16F10 melanoma cells was highest when co-treated with both curcumin and doxorubicin compared to single treatment of either curcumin and doxorubicin. The combined index calculated by medium-effect equation was 0.6069, indicating a synergistic effect. Results of confocal laser scanning microscopy and fluorescence-activated cell sorting corresponded to results from an in vitro cytotoxicity assay, indicating synergistic cytotoxicity induced by both drugs. A C57BL/6 mouse model induced by B16F10 lung metastasis was used to study in vivo therapeutic effects. When curcumin and doxorubicin were simultaneously treated, the metastatic melanoma mass in the lungs macroscopically decreased compared to curcumin or doxorubicin alone. Albumin nanoparticles encapsulating two anticancer drugs were shown to have an effective therapeutic result and would be an excellent way to treat resistant lung cancers. (C) 2017 Elsevier B.V. All rights reserved.