Actinoplanes rhizophilus sp. nov., an actinomycete isolated from the rhizosphere of Sansevieria trifasciata Prain.
Actinoplanes rhizophilus sp. nov., an actinomycete isolated from the rhizosphere of Sansevieria trifasciata Prain.
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DOI：
10.1099/ijsem.0.000646
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发表时间：
2015-12
影响因子：
2.8
通讯作者：
Hairong He;J. Xing;Chongxi Liu;Chuang Li;Zhaoxu Ma;Jian-song Li;W. Xiang;Xiangjing Wang
中科院分区：
文献类型：
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作者：
Hairong He;J. Xing;Chongxi Liu;Chuang Li;Zhaoxu Ma;Jian-song Li;W. Xiang;Xiangjing Wang
从黑龙江省虎尾兰根际土壤中分离到一株放线菌，命名为NEAU-A-2 T。使用多相方法建立了该生物体的分类地位。该菌株在菌丝体上形成不规则的孢子囊，内含活动孢子。全细胞糖是木糖和半乳糖。主要的甲基萘醌是MK-9（H10）、MK-9（H2）、MK-10（H2）和MK-10（H4）。主要脂肪酸为异C15：0、异C16：0和反异C15：0。极性脂质为二磷脂酰甘油、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、三种未鉴别的磷脂和一种未鉴别的糖脂。16 S rRNA基因序列相似性分析表明，菌株NEAU-A-2 T与球孢游动放线菌（Actinoplanes globisporus）NBRC 13912 T的序列相似性最高，属于游动放线菌属（97.7% 16 S rRNA基因序列相似性），锈红游动放线菌IMSNU 22125 T（97.5%）、托文游动放线菌MN 07-A0368 T（97.2%）和里尻游动放线菌NBRC 108556 T（97.2%）;与该属其它种模式菌株的相似性<97%。两种树型构建算法表明，菌株NEAU-A-2 T与A. globisporus NBRC 13912T和A. Rishiriensis NBRC 108556T.然而，较低的DNA-DNA相关值允许分离物与上述两种游动放线菌属物种区分开。此外，菌株NEAU-A-2 T也可以区分与最接近的物种的形态和生理特征。因此，总之，分离物NEAU-A-2 T代表游动放线菌属的一个新种，建议将其命名为嗜根游动放线菌新种。模式菌株为NEAU-A-2 T（= CGMCC 4.7133T = DSM 46672 T）。
A novel actinomycete, designated strain NEAU-A-2T, was isolated from the rhizosphere soil of Sansevieria trifasciata Prain collected from Heilongjiang province, north-east China. The taxonomic status of this organism was established using a polyphasic approach. The isolate formed irregular sporangia containing motile spores on the substrate mycelium. The whole-cell sugars were xylose and galactose. The predominant menaquinones were MK-9(H10), MK-9(H2), MK-10(H2) and MK-10(H4). The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and an unidentified glycolipid. 16S rRNA gene sequence similarity studies showed that strain NEAU-A-2T belongs to the genus Actinoplanes with the highest sequence similarities to Actinoplanes globisporus NBRC 13912T (97.7 % 16S rRNA gene sequence similarity), Actinoplanes ferrugineus IMSNU 22125T (97.5 %), Actinoplanes toevensis MN07-A0368T (97.2 %) and Actinoplanes rishiriensis NBRC 108556T (97.2 %); similarities to type strains of other species of this genus were < 97 %. Two tree-making algorithms showed that strain NEAU-A-2T formed a distinct clade with A. globisporus NBRC 13912T and A. rishiriensis NBRC 108556T. However, low DNA-DNA relatedness values allowed the isolate to be differentiated from the above-mentioned two species of the genus Actinoplanes. Moreover, strain NEAU-A-2T could also be distinguished from the most closely related species by morphological and physiological characteristics. Therefore, in conclusion, isolate NEAU-A-2T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes rhizophilus sp. nov. is proposed. The type strain is NEAU-A-2T ( = CGMCC 4.7133T = DSM 46672T).