Characterization of a Vivid Homolog in Botrytis cinerea
Characterization of a Vivid Homolog in Botrytis cinerea
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DOI：
10.1111/php.12927
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发表时间：
2018-09-01
影响因子：
3.3
通讯作者：
Zoltowski, Brian D.
中科院分区：
文献类型：
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作者：
Foley, Brandon J.;Stutts, Haley;Zoltowski, Brian D.
受光-氧-电压(LOV)家族成员调控的蓝光信号通路整合了胁迫反应、昼夜节律和真菌的致病机制。典型的信号机制包括两个含有LOV的蛋白，它们与粗链霉菌(Neuropora crassa Vivid，NcVVD)和白领1(White Collar1，NcWC1)保持同源性。这些蛋白质参与同源和异源二聚化事件，调节基因转录对光的反应。在此，我们克隆并鉴定了番茄灰霉病菌(Botrytis cinerea，BcVVD)中的VVD同源基因。BcVVD保留了发散的光循环动力学，不能进行LOV介导的同源二聚化，这表明真菌光适应的经典异源/同源二聚化机制发生了改变。
Blue light-signaling pathways regulated by members of the light-oxygen-voltage (LOV) domain family integrate stress responses, circadian rhythms and pathogenesis in fungi. The canonical signaling mechanism involves two LOV-containing proteins that maintain homology to Neurospora crassa Vivid (NcVVD) and White Collar 1 (NcWC1). These proteins engage in homo- and heterodimerization events that modulate gene transcription in response to light. Here, we clone and characterize the VVD homolog in Botrytis cinerea (BcVVD). BcVVD retains divergent photocycle kinetics and is incapable of LOV mediated homodimerization, indicating modification of the classical hetero/homodimerization mechanism of photoadaptation in fungi.