ULTRASTRUCTURAL STUDIES ON CORONET CELLS OF SACCUS VASCULOSUS OF FRESHWATER STICKLEBACK, GASTEROSTEUS-ACULEATUS FORM LEIURUS
ULTRASTRUCTURAL STUDIES ON CORONET CELLS OF SACCUS VASCULOSUS OF FRESHWATER STICKLEBACK, GASTEROSTEUS-ACULEATUS FORM LEIURUS
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DOI：
10.1007/bf00307255
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
BENJAMIN, M
中科院分区：
文献类型：
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作者：
BENJAMIN, M
对淡水棘鱼冠状细胞的电镜研究表明，冠状细胞具有丰富的SER、核周原纤维、微管、游离核糖体和线粒体等特征。冠状细胞内可见酸性磷酸酶阳性的致密体，碱性磷酸酶无活性，且无糖原颗粒。冠状细胞及其微球表面有一层可染钌红物质，在70%的海水中保存1周后，该物质几乎完全消失。在淡水和70%海水中保存1周的定量电镜显示，海水维持鱼的SER小管管腔比淡水维持鱼的SER小管管腔宽。高尔基体所占体积也增加，但线粒体所占细胞质的体积没有变化。冠状细胞的形态和它们对70%海水的反应都支持血管囊是一种渗透（离子）调节结构的假设。
EM studies on the coronet cells of the freshwater stickleback showed that the cells were characterised by abundant SER, perinuclear fibrils, microtubules, free ribosomes and mitochondria. Acid phosphatase-positive dense bodies were present, but there was no alkaline phosphatase activity nor were there any glycogen granules in the coronet cells. On the surface of the coronet cell and its globules was a layer of Ruthenium red-stainable material which almost completely disappeared when the fish were kept in 70% seawater for 1 week. Quantitative EM of fish kept in freshwater or 70% seawater for 1 week showed that the SER tubules of seawater-maintained fish had a wider lumen than those of freshwater-maintained fish. The volume occupied by the Golgi apparatus also increased but there was no change in the volume of the cytoplasm occupied by mitochondria. Both the morphology of the coronet cells and their response to 70% seawater support the hypothesis that the saccus vasculosus is an osmo(iono)regulatory structure.