[Relationship between single nucleotide polymorphisms in IL28B gene and response to interferon treatment in chronic hepatitis B patients].
[Relationship between single nucleotide polymorphisms in IL28B gene and response to interferon treatment in chronic hepatitis B patients].
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发表时间：
2011-12
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通讯作者：
Chu-ming Chen;F. Zhou;Yuanping Zhou;Yanli Zeng;Wei Li-;J. Hou
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作者：
Chu-ming Chen;F. Zhou;Yuanping Zhou;Yanli Zeng;Wei Li-;J. Hou
目的探讨白细胞介素28 B（IL 28 B）基因rs 12979860和rs 8099917单核苷酸多态性（SNPs）与乙型肝炎e抗原（HBeAg）阳性慢性乙型肝炎患者干扰素治疗应答的关系。方法收集82例正在接受干扰素治疗的HBeAg阳性慢性B肝炎患者的外周血样本，其中治疗有效38例，无效44例。从染色体DNA中扩增IL 28 B基因，并基于PCR-RLFP和测序法对rs 12979860 SNP和rs 8099917 SNP进行基因分型。结果在应答组中，SNPrs 8099917基因TT和TG+GG基因型及等位基因G的分布频率分别为81.6%（31/38）、18.4%（7/38）和9.2%（7/76），而SNPrs 8099917基因TT和TG+ GG基因型及等位基因G的分布频率分别为97.7%（43/44）、2.3%（1/44）、2.3%（1/44）和2.3%（1/44）。无反应者为1.1%（1/88）。基因型和等位基因G的频率在反应性和非反应性患者之间显示出显著差异（P=0.014和P=0.025）。SNPrs 12979860位点CT基因型和T等位基因频率在应答组和非应答组间的分布差异无统计学意义（P> 0.05）。结论rs 8099917 SNP可能与HBeAg阳性慢性B型肝炎患者对干扰素治疗的应答相关，等位基因G可能是干扰素治疗成功的预测因子。
OBJECTIVE To explore the relationship between the single nucleotide polymorphisms (SNPs) of rs12979860 and rs8099917 in IL28B gene and the response to interferon treatment in hepatitis B e antigen (HBeAg)-positive patients with chronic hepatitis B patients. METHODS Peripheral blood samples were collected from 82 HBeAg-positive patients with chronic hepatitis B receiving interferon treatment, including 38 with favorable response to the treatment and 44 without response. IL28B gene was amplified from the chromosomal DNA, and rs8099917 SNP was genotyped based on PCR-RLFP and rs12979860 SNP by sequencing. RESULTS In the responsive patients, the distribution frequencies of TT and TG+GG genotypes and allele G in SNPrs8099917 were 81.6% (31/38), 18.4% (7/38), and 9.2% (7/76), as compared to the frequencies of 97.7% (43/44), 2.3% (1/44), and 1.1% (1/88) in nonresponsive patients, respectively. The frequencies showed significant differences between the responsive and nonresponsive patients (P=0.014 for genotypes and P=0.025 for allele G). The distribution frequencies of CT genotypes and allele T in SNPrs12979860 showed no differences between the responsive and nonresponsive patients (P<0.05). CONCLUSION rs8099917 SNP is probably associated with the response to interferon treatment in HBeAg-positive patients with chronic hepatitis B, and Allele G may be predictive of the treatment success.