A simplified method of generating transgenic Xenopus.
A simplified method of generating transgenic Xenopus.
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DOI：
10.1093/nar/28.4.e12
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发表时间：
2000-02-15
影响因子：
14.9
通讯作者：
Mohun, T J
中科院分区：
文献类型：
--
作者：
Sparrow, D B;Latinkic, B;Mohun, T J
目前，转基因青蛙胚胎是通过限制性酶介导的整合（REMI）在解压缩的精子核上产生的，然后将核移植到未受精卵中。我们开发了该协议的简化版本，有可能增加正常发育的转基因胚胎的数量。
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.