FgMon1, a guanine nucleotide exchange factor of FgRab7, is important for vacuole fusion, autophagy and plant infection in Fusarium graminearum.
FgMon1, a guanine nucleotide exchange factor of FgRab7, is important for vacuole fusion, autophagy and plant infection in Fusarium graminearum.
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FgMon1 是 FgRab7 的鸟嘌呤核苷酸交换因子，对于禾谷镰刀菌的液泡融合、自噬和植物感染非常重要
DOI：
10.1038/srep18101
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发表时间：
2015-12-10
影响因子：
4.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li Y;Li B;Liu L;Chen H;Zhang H;Zheng X;Zhang Z
Ccz 1-Mon 1蛋白复合物，即晚期内体Rab 7同源物Ypt 7的鸟嘌呤核苷酸交换因子（GEF），是多个空泡递送途径的晚期步骤所必需的，例如细胞质到空泡靶向（Cvt）途径和自噬过程。在这里，我们确定并表征了禾谷镰刀菌中的酵母Mon 1同源物，命名为FgMon 1。FgMON 1编码一种运输蛋白，在丝状真菌中高度保守。目的基因的缺失表明，该突变体在营养生长、无性/有性发育、分生孢子萌发和形态、植物侵染和脱氧雪腐镰刀菌烯醇产生等方面存在缺陷。细胞学检查显示，Fgmon 1突变体也有缺陷的空泡融合和自噬和内吞延迟。酵母双杂交和体外GST-pull down实验证实FgMon 1与Rab GT3 FgRab 7相互作用，而Rab GT3 FgRab 7在F.禾谷早熟禾FgMon 1可能充当FgRab 7的GEF，并且组成性激活的FgRab 7能够挽救FgMon 1突变体的缺陷。综上所述，我们的研究证明了FgMon 1和FgRab 7是调节囊泡运输、内吞和自噬的关键组分，从而影响了F.禾谷早熟禾
The Ccz1-Mon1 protein complex, the guanine nucleotide exchange factor (GEF) of the late endosomal Rab7 homolog Ypt7, is required for the late step of multiple vacuole delivery pathways, such as cytoplasm-to-vacuole targeting (Cvt) pathway and autophagy processes. Here, we identified and characterized the yeast Mon1 homolog in Fusarium graminearum, named FgMon1. FgMON1 encodes a trafficking protein and is well conserved in filamentous fungi. Targeted gene deletion showed that the ∆Fgmon1 mutant was defective in vegetative growth, asexual/sexual development, conidial germination and morphology, plant infection and deoxynivalenol production. Cytological examination revealed that the ∆Fgmon1 mutant was also defective in vacuole fusion and autophagy and delayed in endocytosis. Yeast two hybrid and in vitro GST-pull down assays approved that FgMon1 physically interacts with a Rab GTPase FgRab7 which is also important for the development, infection, membrane fusion and autophagy in F. graminearum. FgMon1 likely acts as a GEF of FgRab7 and constitutively activated FgRab7 was able to rescue the defects of the ∆Fgmon1 mutant. In summary, our study provides evidences that FgMon1 and FgRab7 are critical components that modulate vesicle trafficking, endocytosis and autophagy and thereby affect the development, plant infection and DON production of F. graminearum.