Direct MS/MS analysis in mammalian tissue sections using MALDI‐QIT‐TOFMS and chemical inkjet technology
Direct MS/MS analysis in mammalian tissue sections using MALDI‐QIT‐TOFMS and chemical inkjet technology
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DOI：
10.1002/sia.2389
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发表时间：
2006-12
影响因子：
1.7
通讯作者：
S. Shimma;M. Furuta;K. Ichimura;Yoshikazu Yoshida;M. Setou
中科院分区：
文献类型：
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作者：
S. Shimma;M. Furuta;K. Ichimura;Yoshikazu Yoshida;M. Setou
二维样品的质谱分析被称为成像质谱分析，它使我们能够可视化组织表面的分子分布。这项技术是利用基质辅助激光解吸/电离飞行时间质谱仪（MALDI - TOFMS）开发的。然而，很难确定组织切片上感兴趣区域的成分。我们开发了一种方法来克服这个问题。我们将其命名为组织消化。采用化学喷墨技术，应用酶和基质辅助激光解吸/电离-四极离子阱-飞行时间串联质谱仪（MALDI - QIT - TOFMS）直接识别组织表面的分子。这项技术为我们深入了解生物结构，特别是蛋白质的复合材料提供了一种工具。版权所有©2006约翰威利父子有限公司
Mass spectrometry on 2D samples is known as imaging mass spectrometry, which enables us to visualize molecular distribution on a tissue surface. This technology has been developed using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometers (MALDI‐TOFMS). However, it is difficult to determine constituents of interested regions on the tissue sections. We developed the methodology to overcome this problem. We designated it on‐tissue digestion. It is performed with the chemical inkjet technology to apply enzymes and the matrix‐assisted laser desorption/ionization‐quadrupole ion trap‐time‐of‐flight tandem mass spectrometer (MALDI‐QIT‐TOFMS) to identify molecules directly on the tissue surface. This technique provided us with a tool for an in‐depth understanding of composites of biostructures, especially proteins. Copyright © 2006 John Wiley & Sons, Ltd.