Overexpressing GSH1 and AsPCS1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in Arabidopsis thaliana
Overexpressing GSH1 and AsPCS1 simultaneously increases the tolerance and accumulation of cadmium and arsenic in Arabidopsis thaliana
复制标题
过表达GSH1和AsPCS1同时增加拟南芥对镉和砷的耐受性和积累
DOI：
10.1016/j.chemosphere.2008.04.018
复制
发表时间：
2008-07-01
期刊：
影响因子：
8.8
通讯作者：
Ma, Mi
中科院分区：
文献类型：
--
作者：
Guo, Jiangbo;Dai, Xiaojing;Ma, Mi
本研究的目的是通过在拟南芥中同时过表达AsPCS1和GSH1（来自大蒜和面包酵母）来开发具有增加的耐受性和从土壤中积累重金属和类金属的转基因植物。植物螯合肽（Phytochelatins，PC）和谷胱甘肽（Glutathione，GSH）是植物和其他生物体内参与重金属离子螯合作用的主要结合肽。单基因转基因株系比野生型具有更高的耐性和积累更多的镉和砷。与单基因转基因株系相比，双基因转基因株系表现出更高的Cd和As耐性和积累量。其中一个双基因转基因株系PG1积累的Cd量是单基因转基因株系的两倍。同时过表达AsPCS1和GSH1导致转基因拟南芥中总PC产量升高。这些结果表明，这种堆叠的修饰基因是能够增加镉和砷的耐受性和积累的转基因株系，并代表了一个非常有前途的新工具，用于植物修复的努力。(C)2008爱思唯尔有限公司版权所有。
The goal of this study was to develop transgenic plants with increased tolerance for and accumulation of heavy metals and metalloids from soil by simultaneous overexpression of AsPCS1 and GSH1 (derived from garlic and baker's yeast) in Arabidopsis thaliana. Phytochelatins (PCs) and glutathione (GSH) are the main binding peptides involved in chelating heavy metal ions in plants and other living organisms. Single-gene transgenic lines had higher tolerance to and accumulated more Cd and As than wild-type. Compared to single-gene transgenic lines, dual-gene transformants exhibited significantly higher tolerance to and accumulated more Cd and As. One of the dual-gene transgenic lines, PG1, accumulated twice the amount of Cd as single-gene transgenic lines. Simultaneous overexpression of AsPCS1 and GSH1 led to elevated total PC production in transgenic Arabidopsis. These results indicate that such a stacking of modified genes is capable of increasing Cd and As tolerance and accumulation in transgenic lines, and represents a highly promising new tool for use in phytoremediation efforts. (C) 2008 Elsevier Ltd. All rights reserved.