A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
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DOI：
10.3791/53042
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发表时间：
2015-10-01
影响因子：
1.2
通讯作者：
Goodlad, Robert A.
中科院分区：
文献类型：
--
作者：
Yoneda, Mitsuhiro;Molinolo, Alfredo A.;Goodlad, Robert A.
为随后的分析或定量准备完整的小鼠小肠和结肠可能既耗时又困难。我们描述了使用一种简单的设备来切割和‘卷起’小鼠的肠道，以快速制备质量优于手工所能达到的质量和均匀的整装制剂。该装置包括一个容纳4根不锈钢棒的底座和一个起切割导向作用的顶部。这些杆状物被插入小肠的管腔[分为三分之一]和结肠。然后，将棒和样品放在一张滤纸或卡片上，放入设备底座上的保持槽中。然后，设备的顶部被定位并用作切割指南。顶片中心的两个成角度的部分用来引导刀或手术刀，并在杆子的顶部纵向切割肠道。一旦切开肠道部分，取下顶部，将卡片、组织和棒从设备中轻轻取出并放置在长凳上。然后轻轻地将杆子侧向滚动，使肠段变平，并将其粘在下面的滤纸或卡片上。然后可以检查或固定最后的制备并存储以供以后分析。这些制剂对于研究正常或转基因小鼠模型的肠道变化是非常有价值的。这些制剂已被用于研究和量化炎症(结肠炎)、损伤、癌前病变(异常隐窝灶(ACFS)和粘蛋白耗竭灶(MDFS))以及息肉或肿瘤的影响。
Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe the use of a simple device to cut and 'roll' mouse intestines to rapidly prepare whole mount preparations of superior and uniform quality to that which can be achieved by hand. The device comprises a base that holds 4 stainless steel rods and a top, which acts a cutting guide. The rods are inserted into the lumen of the small intestine [divided into thirds] and the colon. The rods and samples are then placed over a piece of filter paper or card into the holding slots in the base of the device. The top of the device is then positioned and serves as a cutting guide. The two angled sections in the center of the top piece are used to guide a knife or scalpel and cut the intestines longitudinally on the top of the rods. Once the intestinal sections have been cut, the top is removed and the card, tissue and rods gently removed from the device and placed on the bench. The rods are then gently rolled sideways to flatten and stick the intestinal segments onto the underlying piece of filter paper or card. The final preparation can then be examined or fixed and stored for later analysis. The preparations are invaluable for the study of intestinal changes in normal or genetically modified mouse models. The preparations have been used for the study and quantification of the effects of inflammation (colitis), damage, pre-cancerous lesions (aberrant crypt foci (ACFs) and mucin depleted foci (MDFs)) and polyps or tumors.