Determination of 5'-nucleotidase activity in human erythrocytes and plasma using high-performance liquid chromatography.
Determination of 5'-nucleotidase activity in human erythrocytes and plasma using high-performance liquid chromatography.
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使用高效液相色谱法测定人红细胞和血浆中的 5-核苷酸酶活性。
DOI：
10.1016/s0021-9673(00)82031-2
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
K. Ushio
中科院分区：
文献类型：
--
作者：
T. Sakai;S. Yanagihara;K. Ushio
本文介绍一种测定人红细胞和血浆中5‘-核苷酸酶活性的方法。采用反相高效液相色谱法，在不到4min的时间内将产物(尿苷)与底物(尿苷-5‘-单磷酸)分离。测定的活度与传统的测定无机磷释放的方法测定的活度非常一致。本方法不需要在测定前对酶溶液进行透析，并且与其他测定方法相比具有几个优点，包括高灵敏度。
A method is described for the determination of 5′-nucleotidase activity in human erythrocytes and plasma. Using reversed-phase high-performance liquid chromatography; the product (uridine) was separated from the substrate (uridine-5′-monophosphate) in less than 4 min. The activity determined closely agreed with that determined by the conventional method, in which the inorganic phosphate released is measured. The present method eliminates the need for dialysis of enzyme solution prior to the assay, and offers several advantages over other assay methods, including high sensitivity.