Sodium gradient dependence of proline and glycine uptake in rat renal brush-border membrane vesicles.
Sodium gradient dependence of proline and glycine uptake in rat renal brush-border membrane vesicles.
复制标题
大鼠肾刷状缘膜囊泡脯氨酸和甘氨酸摄取的钠梯度依赖性。
DOI：
10.1016/0005-2736(79)90427-9
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
S. Segal
中科院分区：
文献类型：
--
作者：
P. McNamara;L. Pepe;S. Segal
本实验观察了脯氨酸和甘氨酸进入大鼠肾刷状缘膜囊泡的钠依赖性。脯氨酸的高Km系统显示无钠依赖性。甘氨酸进入的lowKm系统严格依赖于Na+梯度，但没有证据表明载体系统对Na+具有任何亲和力。脯氨酸转运的低Km系统和甘氨酸转运的高Km系统似乎是共享的。这两个系统都受到Na+梯度的刺激，似乎对Na+具有亲和力。在离子梯度中降低Na+浓度的作用是改变氨基酸进入的Km，并且在低Na+浓度下抑制甘氨酸进入的V。
The sodium-dependent entry of proline and glycine into rat renal brushborder membrane vesicles was examined. The highKmsystem for proline shows no sodium dependence. The lowKmsystem for glycine entry is strictly dependent on a Na+gradient but shows no evidence of the carrier system having any affinity for Na+. The lowKmsystem for proline and highKmsystem for glycine transport appear to be shared. Both systems are stimulated by a Na+gradient and appear to have an affinity for the Na+. The effect of decreasing the Na+concentration in the ionic gradient is to alter theKmfor amino acid entry and, at low Na+concentrations, to inhibit theVfor glycine entry.