INTERORGAN REGULATION OF ETHYLENE BIOSYNTHETIC GENES BY POLLINATION
INTERORGAN REGULATION OF ETHYLENE BIOSYNTHETIC GENES BY POLLINATION
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DOI：
10.1105/tpc.5.4.419
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发表时间：
1993-04-01
期刊：
影响因子：
11.6
通讯作者：
HALEVY, AH
中科院分区：
文献类型：
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作者：
ONEILL, SD;NADEAU, JA;HALEVY, AH
花粉症引发一系列有助于成功繁殖的发育事件，包括花被衰老、色素沉着变化和胚珠分化，为即将到来的受精做准备。在兰花中，这些过程中的每一个在不同的花器官的启动是严格和协调控制授粉，从而提供了一个独特的机会来研究协调器官间授粉后发展的信号。由于乙烯已被牵连在促进授粉后发育的几个方面的调节，我们专注于确定其生物合成基因的表达和它们在调节中可能发挥的作用。在柱头、子房和唇瓣中，1-氨基环丙烷-1-羧酸（ACC）合酶和ACC氧化酶的mRNA表达量受去雄、生长素和乙烯的协同调控。虽然花瓣贡献了高达26%的总花乙烯和积累高水平的ACC氧化酶的mRNA和活性授粉后，没有ACC合酶的mRNA或活性检测在这个组织中。总之，这些结果支持一个模型的器官间调节授粉后的发展，这取决于授粉刺激的积累的mRNA编码乙烯blosynthetic酶的发育调节和组织特异性的方式。该模型依赖于可溶性激素前体ACC的易位，而不是激素本身的易位。以这种方式，ACC用于启动已经由花的其他部分感知的乙烯启动的反应。因此，ACC可能阿萨种次级传递信号，协调不同花器官的授粉后发育。
Pollination initiates a syndrome of developmental events that contribute to successful reproduction, including perianth senescence, changes in pigmentation, and ovule differentiation in preparation for impending fertilization. In orchid flowers, initiation of each of these processes in distinct floral organs is strictly and coordinately controlled by pollination, thus providing a unique opportunity to study the signals that coordinate interorgan postpollination development. Because ethylene has been implicated in contributing to regulation of several aspects of postpollination development, we focused on determining the expression of its biosynthetic genes and their possible role in regulation. The abundance of mRNA encoding both 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase in the stigma, ovary, and labellum was found to be coordinately regulated by emasculation, auxin, and ethylene. Although petals contribute up to 26% of total flower ethylene and accumulate high levels of ACC oxidase mRNA and activity following pollination, no ACC synthase mRNA or activity was detected in this tissue. Together, these results support a model of interorgan regulation of postpollination development that depends on pollination-stimulated accumulation of mRNA encoding ethylene blosynthetic enzymes in a developmentally regulated and tissue-specific manner. This model relies on the translocation of a soluble hormone precursor, ACC, rather than on the translocation of the hormone itself. In this way, ACC serves to actuate the response already initiated by ethylene perceived by other parts of the flower. Thus, ACC may function asa secondary transmissible signal that coordinates postpollination development in diverse floral organs.