Involvement of putative chemical wound signals in the induction of phenolic metabolism in wounded lettuce
Involvement of putative chemical wound signals in the induction of phenolic metabolism in wounded lettuce
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DOI：
10.1034/j.1399-3054.2002.1140111.x
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发表时间：
2002-01-01
影响因子：
6.4
通讯作者：
Saltveit, ME
中科院分区：
文献类型：
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作者：
Campos-Vargas, R;Saltveit, ME
切割长叶生菜（Lactuca sativa L. cv. Longifolia）的叶子会产生伤口信号，诱导苯丙氨酸解氨酶（PAL，EC 4.3.1.5）的合成，以及酚类化合物在细胞中从损伤部位开始长达 2 小时的积累，以及损伤部位附近的组织褐变。长叶生菜头上的叶子对假定的化学伤口信号[脱落酸（ABA）、茉莉酸（JA）和茉莉酸甲酯（MeJA）]的反应随叶龄的不同而显着不同。将收获的生菜头暴露于 ABA、JA、MeJA 或水杨酸 (SA) 中不会引起 PAL 活性、酚类化合物浓度的变化，也不会导致成熟叶组织褐变，这与受伤引起的水平相似。在 5 或 10℃ 下以蒸气（10、100 或 1000 mul kg(-1) FW）或水喷雾或浸液（0.01-100 muM）形式施用茉莉酸甲酯，不会对 PAL 活性或褐变产生影响，这与未处理的对照有显着差异。相比之下，JA、MeJA 和 SA 确实诱导嫩叶中 PAL 活性水平升高。然而，诱发的水平远低于受伤诱发的水平。成熟叶子中伤口诱导的酚类代谢似乎是由与幼叶中功能不同的信号诱导的。
Cutting leaves of Romaine lettuce (Lactuca sativa L. cv. Longifolia) produces a wound signal that induces the synthesis of phenylalanine ammonia lyase (PAL, EC 4.3.1.5) and the accumulation of phenolic compounds in cells up to 2 ern from the site of injury, and tissue browning near the site of injury. The response of leaves within a head of Romaine lettuce to putative chemical wound signals [abscisic acid (ABA), jasmonate (JA) and methyl jasmonate (MeJA)] differed significantly with leaf age. Exposure of harvested heads of lettuce to ABA, JA, MeJA, or salicylic acid (SA) did not induce changes in PAL activity, the concentration of phenolic compounds or browning in mature leaf tissue that was similar to the level induced by wounding. Methyl jasmonate applied as vapour (10, 100 or 1000 mul kg(-1) FW), or as an aqueous spray or dip (0.01-100 muM) at 5 or 10degreesC did not produce an effect on PAL activity or browning that differed significantly from the untreated controls. In contrast, JA, MeJA and SA did induce elevated levels of PAL activity in younger leaves. However, the levels induced were far lower than those induced by wounding. Wound induced phenolic metabolism in mature leaves appears to be induced by different signals than those functioning in young leaves.