Aggregate Structure in Dilute Aqueous Dispersions of Phospholipids, Fatty Acids, and Lysophospholipids
Aggregate Structure in Dilute Aqueous Dispersions of Phospholipids, Fatty Acids, and Lysophospholipids
复制标题
磷脂、脂肪酸和溶血磷脂稀水分散体中的聚集体结构
作者：
Nill Bergstrand and;K. Edwards
采用冷冻透射电子显微镜 (cryo-TEM) 研究鸡蛋磷脂酰胆碱 (EPC)、油酸 (OA) 和溶血磷脂单油酰磷脂酰胆碱 (MOPC) 的稀水分散体中的聚集体结构。在生理pH和盐浓度下，在含有等摩尔浓度的三种组分的混合物中检测到相对单分散的单层脂质体群体。在含有高浓度 MOPC 的样品中，线状胶束构成了主要的聚集结构。另一方面，过量的脂肪酸迫使系统转向具有净负曲率的结构。在没有磷脂的情况下，冷冻透射电镜显示，在含有相同摩尔量的 MOPC 和 OA 的混合物中，双层碎片与线状胶束共存。将 MOPC 外部添加到预制的 EPC 脂质体中会导致中间结构（包括开放脂质体和双层片段）的浓度依赖性演化。结构改造...
Cryo-transmission electron microscopy (cryo-TEM) was employed to investigate the aggregate structure in dilute aqueous dispersions of egg-phosphatidylcholine (EPC), oleic acid (OA), and the lysophospholipid monooleoylphosphatidylcholine (MOPC). At physiological pH and salt concentration, a relatively monodisperse population of unilamellar liposomes was detected in mixtures containing equimolar concentrations of the three components. Threadlike micelles constituted the dominant aggregate structure in samples containing high concentrations of MOPC. Excess fatty acid forced, on the other hand, the system toward structures with net negative curvature. In the absence of phospholipid, cryo-TEM revealed bilayer fragments in coexistence with threadlike micelles in mixtures containing the same molar amount of MOPC and OA. External addition of MOPC to preformed EPC liposomes gave rise to a concentration dependent evolution of intermediate structures, including open liposomes and bilayer fragments. The structural re...