A local coupling model and compass parameter for eukaryotic chemotaxis
A local coupling model and compass parameter for eukaryotic chemotaxis
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DOI：
10.1016/j.devcel.2004.12.007
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发表时间：
2005-02-01
影响因子：
11.8
通讯作者：
Meyer, T
中科院分区：
文献类型：
--
作者：
Arrieumerlou, C;Meyer, T
趋化性是一种细胞感应机制，它引导免疫细胞到达感染部位，并将成纤维细胞引导到损伤部位。在这里，我们表明，在迁移的初级树突状细胞和成纤维细胞的前沿是不是一个统一的信号实体，而是由独立的耦合单元，其中PI3激酶的瞬时激活链接到本地的片状延伸和小离散转向的方向迁移。这些发现导致了一个模型，其中全球细胞极化是独立的趋化机制。在该模型中，趋化性不需要空间整合，而是一个随机过程，其中前缘内的每个受体结合事件触发局部片足延伸和迁移方向的小转弯。我们发现，这个模型和派生的“罗盘参数”是足够的模拟观察到的随机迁移，有偏见的随机游走，和持久的真核细胞的趋化行为。
Chemotaxis is a cellular sensing mechanism that guides immune cells to sites of infection and leads fibroblasts to sites of injury. Here, we show in migrating primary dendritic cells and fibroblasts that the leading edge is not a uniform signaling entity, but instead consists of independent coupling units in which transient activation of PI3-kinase links to local lamellipod extension and small discrete turns in the direction of migration. These findings led to a model in which global cell polarization is independent from the chemotaxis mechanism. In this model, chemotaxis does not require spatial integration but is instead a stochastic process in which each receptor binding event within the leading edge triggers a local lamellipod extension and a small turn in the direction of migration. We show that this model and a derived "compass parameter" are sufficient to simulate the observed random migration, biased random walk, and persistent chemotactic behaviors of eukaryotic cells.