X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells.
X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells.
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DOI：
10.18632/oncotarget.12111
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发表时间：
2016-11-01
期刊：
影响因子：
--
通讯作者：
Sun XF
中科院分区：
文献类型：
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作者：
Blockhuys S;Liu N;Agarwal NR;Enejder A;Loitto V;Sun XF
直肠癌治疗仍然失败，局部和远处复发。据推测，放射治疗可以刺激癌细胞扩散和转移。在本研究中，我们评估了 X 辐射对 I 型胶原带形成潜力（即与间充质细胞迁移相关的基质重塑）以及 SW480、SW620、HCT116 p53+/+ 和 HCT116 p53−/− 结肠癌细胞行为的影响。我们确定了辐射诱导的 I 型胶原带形成以及 SW480 和 HCT116 p53+/+ 迁移潜力的增加。对 HCT116 p53+/+ 的进一步研究表明，X 辐射带形成后细胞的运动性增强。此外，我们检测到 X 辐射后 HCT116 p53+/+ 的粘附潜力和成熟整合素 β1 表达降低，但非肌肉肌球蛋白 II 表达没有变化。在假手术和 X 辐射条件下，整合素 β1 中和导致细胞粘附和 I 型胶原带形成减少。我们的研究表明，I 型胶原带形成是结肠癌细胞 X 辐射后迁移潜力增加的潜在机制，并表明除整合素 β1 和非肌肉肌球蛋白 II 之外的其他分子是辐射诱导结肠癌细胞 I 型胶原带形成潜力的原因。这项工作鼓励对辐射引起的迁移进行进一步的分子研究，以改善直肠癌的治疗结果。
Rectal cancer treatment still fails with local and distant relapses of the disease. It is hypothesized that radiotherapy could stimulate cancer cell dissemination and metastasis. In this study, we evaluated the effect of X-radiation on collagen type I strap formation potential, i.e. matrix remodeling associated with mesenchymal cell migration, and behaviors of SW480, SW620, HCT116 p53+/+ and HCT116 p53−/− colon cancer cells. We determined a radiation-induced increase in collagen type I strap formation and migration potentials of SW480 and HCT116 p53+/+. Further studies with HCT116 p53+/+, indicated that after X-radiation strap forming cells have an increased motility. More, we detected a decrease in adhesion potential and mature integrin β1 expression, but no change in non-muscle myosin II expression for HCT116 p53+/+ after X-radiation. Integrin β1 neutralization resulted in a decreased cell adhesion and collagen type I strap formation in both sham and X-radiated conditions. Our study indicates collagen type I strap formation as a potential mechanism of colon cancer cells with increased migration potential after X-radiation, and suggests that other molecules than integrin β1 and non-muscle myosin II are responsible for the radiation-induced collagen type I strap formation potential of colon cancer cells. This work encourages further molecular investigation of radiation-induced migration to improve rectal cancer treatment outcome.